Effects of diets containing selenium-enriched Lactobacillus plantarum on heavy metal bioaccumulation and oxidative stress in tissues of bulatmai barbel (Luciobarbus capito) exposed to lead stress
ZHANG Qing, SHANG Xinchi, GENG Longwu, LI Haitao, DU Rujun, CONG Yanfeng, XU Wei*
1.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 2.Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Harbin 150070, China; 3.Duerbot Mongolian Autonomous County Fisheries Station of Heilongjiang Province, Daqing 166299, China
Abstract: In order to explore the protective effect of selenium-enriched Lactobacillusplantarum on bulatmai barbel (Luciobarbuscapito) under lead (Pb) stress, bulatmai barbel with body weight of (75.4±0.9)g was reared in a 90 cm×55 cm×55 cm fiberglass tank, exposed to tap water (control group), and tap water containing 0.2 mg/L Pb from Pb(CH3COO)2·3H2O (group Pb), and a basic diet containing nano-selenium prepared from MRS liquid medium containing sodium selenite (Na2SeO3, 200 μg/mL) and L.plantarum by aerobic static culture for 24 hours, with a dose of 5 mg/kg and L.plantarum at dose of 3.125×108 CFU/kg (group SL+ Pb) at water temperature of (23±2)℃. The accumulation of selenium and lead, and activities of antioxidant enzymes were determined in liver, kidney, gill, intestine and muscle, and the expression of antioxidant genes were detected by Real-time PCR. The results showed that when the sodium selenite was 200 μg/mL, the number of colonies of L.plantarum was up to 1.150×107 CFU/mL, the nano-selenium content was 184 μg/mL, and spherical nano-selenium particles (SeNPs) were observed under an electronic microscope. There was significantly higher accumulation of selenium in the selenium-enriched lead group than that in the control group and the lead group (P<0.05). There were significantly lower selenium contents in liver, kidney, gill, and muscle in the lead group than that in the control group (P<0.05), with significantly higher in intestine than that in the control group (P<0.05). The descending order of accumulation levels of lead in different tissues of the fish in the lead group was expressed as gills> livers> kidneys> intestines> muscles, with significantly lower in selenium-enriched lead group than in the lead group (P<0.05). The selenium-enriched L.plantarum led to significantly increase the activities of catalase, superoxide dismutase and glutathione peroxidase, to significantly decrease the content of malondialdehyde in kidneys and gills tissues under lead stress (P<0.05). There were significantly decrease in relative expression levels of Nrf2, HO-1 and GPx in kidneys in the lead group and the selenium-enriched lead group compared with the lead group (P<0.05), and the above indices in the selenium-enriched lead group were found to be significantly increased (P<0.05). In conclusion, adding selenium-enriched L.plantarum to the feed led to reduce the lead accumulation in various tissues and to alleviate the oxidative stress and the expression levels of genes in the fish under lead stress.
张晴, 尚信池, 耿龙武, 李海涛, 杜汝君, 丛艳锋, 徐伟. 富硒植物乳杆菌对大鳞鲃组织铅积累及氧化应激的影响[J]. 大连海洋大学学报, 2023, 38(1): 43-50.
ZHANG Qing, SHANG Xinchi, GENG Longwu, LI Haitao, DU Rujun, CONG Yanfeng, XU Wei. Effects of diets containing selenium-enriched Lactobacillus plantarum on heavy metal bioaccumulation and oxidative stress in tissues of bulatmai barbel (Luciobarbus capito) exposed to lead stress. Journal of Dalian Ocean University, 2023, 38(1): 43-50.
2023, Vol. 38 
