Histopathological observation of gas bubble disease in Pacific white leg shrimp Litopenaeus vannamei
QI Ruirong, TANG Shaolin, ZHANG Jipeng, CUI Longbo, MA Jiahao
1.Guangzhou Liyang Aqua-Technology Company Limited, Guangzhou 510515, China; 2. Guangzhou Jinshui Animal Health Products Company Limited, Guangzhou 510515, China; 3.College of Life Sciences, Yantai University, Yantai 264005, China
Abstract: In order to probe Pacific white leg shrimp Litopenaeusvannamei with gas bubble disease naturally occurring and with body length of (6.3±1.5)cm in an earthen pond, histopathological changes were primarily investigated by field microscopy, bacterial isolation and culture, PCR detection and histological techniques. The results showed that the diseased shrimp had slightly red body in color, and white abdominal muscle segments. The air column and bubbles were observed in the appendages, gill, antennae, and the muscles of cephalothorax. The pathological features of the diseased tissues were characterized by bubbles in muscle, eyes, gills, hepatopancreas, antennal and heart tissues. The bubbles were concentrated in the middle and posterior segments of the muscle, also found in the dorsal ventral vessels and branchial axis vessels. There were more and bigger bubbles in the shrimp with obvious bubbles in clinical than the samples with invisible bubbles. The bubbles in the gill became more and bigger with dissolved oxygen concentrations increase in dissolved oxygen varying from 4.15 mg/L to 15.06 mg/L (saturation of 56%-293%); the eyes showed vacuolation, one side eye lesions were slightly heavier at low level of dissolved oxygen, whereas vacuolization became serious when dissolved oxygen value was increased. meanwhile serious vacuolization were observed in both sides of the eye at high level of dissolved oxygen. Findings indicated that the above phenomena or pathological characteristics were diagnosed as gas bubble disease of Pacific white leg shrimp.
2021, Vol. 36 
