Synergistic effects of high tem perature and low salinity on immunity of M anila clam Ruditapes philippinarum
YANG Dong-min,ZHANG Yan-li,DING Jian-feng,YANG Feng, HUO Zhong-ming,NIE Hong-tao,YAN Xi-wu
College of Fisheries and Life Science,Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,Dalian Ocean University, Dalian 116023,China
Abstract: The changes in blood cell count,phagocytosis ability,osmotic pressure,and activities of lysozyme (LZM),and alkaline phosphatase(AKP)in serum were monitored in Manila clam Ruditapes philippinarum with body weight of(14.8依0.73)g exposed to temperatures(21益,and 30益)and a salinity of7,15,and 32 for 0, 3,6,12,24,48,and 72 h in order to investigate the synergistic effects of temperatures and salinity on the immuni-ty of Manila clam.The results showed that therewere some fluctuations in blood parameters and activities of serum LZM and AKP in the experimental groups at water temperature of 21益and a salinity of 32,without significant differences(P>0.05)in 0-72 h compared with initial level in the control group.The blood cells countswere re-covered to the initial level in the clam in the 21益and low salinity of7 group in 72 h,while the blood cells counts were not returned to the initial level in the clam in the 30益,32益and salinity 32 groups in 72 h.The clam in high temperature 30益and low salinity 7 group died in 48 h,although they had recovery blood cell count in 24 h. The clam in high temperature 30益and low salinity 7 showed elevated phagocytosis firstand then decrease in hago-cytosisd,even lower than the initial level in 72 h(P<0.05),while the clam inmedium salinity 15 and 7 and 21益groups had recovery phagocytic activities,with higher phagocytosis in high temperature 30益and low salinity 7 groups in 24 h(P<0.05).There were significantly higher LZM activities than the initial level in the clam among experimental groups in 72 h(P<0.05),with themaximal AKP activity in 3 h and recovery initial levels in 72 h. The gradual decrease in osmotic pressure was observed in medium and low salinity groups,while the gradual in-crease in osmotic pressure in the high temperature 30益and salinity 32 group,without recovery initial level in 72 h.The findings indicate that the interaction of temperatures increase and salinity decrease will have amore serious effect on the immunity of shellfish,which may be involved in the high mortality ofmudflat shellfish in summer.
杨东敏, 张艳丽, 丁鉴锋, 杨凤, 霍忠明, 聂鸿涛, 闫喜武. 高温、 低盐对菲律宾蛤仔免疫能力的影响[J]. 大连海洋大学学报, 2017, 32(3): 302-309.
YANG Dong-min, ZHANG Yan-li, DING Jian-feng, YANG Feng, HUO Zhong-ming, NIE Hong-tao, YAN Xi-wu. Synergistic effects of high tem perature and low salinity on immunity of M anila clam Ruditapes philippinarum. Journal of Dalian Ocean University, 2017, 32(3): 302-309.
2017, Vol. 32 
