Identification and drug sensitivity of a pathogenic Vibrio vulnificus isolated from pearl gentian，a hybrid of brownmarbled grouper Epinephelus fuscoguttatus♀×E.lanceolatus♂
1.Key Laboratory of South China Sea Fishery Resouurce Exploitation&Utilization，Ministry of Agriculture，South China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510300，China；2.College of Fisheries and Life Sciences，Shanghai Ocean University，Shanghai201306，China；3.Tropical Fisheries Research and DevelopmentCenter，South China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Sanya 572018，China
Abstract: A bacterium strain X11XC26 was isolated from pearl gentian，a hybrid of brownmarbled Epinephelus fuscoguttatus♀×E.lanceolatus♂，whose virulence to grouper Epinephelus coioides was confirmed by pathogenicity assays，with LD50of 1.70×105CFU/g in the fishes infected by intraperitoneal injection.The physiological test revealed that it had significantly similar characteristics to Vibrio vulnificus.The phylogenetic analysis of 16S rDNA，rctB，topA，mreB and rpoD genes revealed that the strain X11XC26 showed the high similarity to V.vulnificus.Itwas found that the strain X11XC26 was sensitive to norfloxacin，ofloxacin，ciprofloxacin，enrofloxacin and amoxicilin，and not sensitive to polymyxin K-B.The strain X11XC26 of V.vulnificus isolated for the first time from pearl gentian is not reported until now，and should be paid more attention to its drug resistance.
辜良斌, 徐力文, 王雨, 苏友禄, 刘广锋, 郭志勋, 冯娟. 从珍珠龙趸体内分离的1株创伤弧菌的鉴定及耐药性分析[J]. 大连海洋大学学报, 2016, 31(2): 162-167.
GU Liang-bin, XU Li-wen, WANG Yu, SU You-lu, LIU Guang-feng, GUO Zhi-xun, FENG Juan. Identification and drug sensitivity of a pathogenic Vibrio vulnificus isolated from pearl gentian，a hybrid of brownmarbled grouper Epinephelus fuscoguttatus♀×E.lanceolatus♂. Journal of Dalian Ocean University, 2016, 31(2): 162-167.
2016, Vol. 31 
