Karyotype and Ag-NORs of sailfin molly Poecilia latipinna
WANG Lin-chao,BAO Hai-gang,LIJun-ying,WU Chang-xin
Laboratory of Animal Genetic Resource and Molecular Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China
Abstract: Themetaphase chromosome spreadingwas conducted in gills and fins cells of sailfinmolly Poecilia latipinna by colchicine-low concentration-hot film drops,and Ag-NORs were analyzed to obtain cytogenetic data of sailfin molly.The results showed that the karyotype of sailfin molly composed of 2n=46t,NF=46.Ag-NORswere found on the end of chromosome No.2 and chromosome No.9,and the Ag-NORs polymorphismswere individually specific,while no sex chromosome was observed in this fish.The findingswill provide reference to genetic pattern of sailfinmolly.