The antitumor effect of intestinal polysaccharides in sea cucumber Apostichopus japonicus
WANG Bin1,YUAN Tian1,ZHANG Yi-wen2,WANG Zhi-yuan1, QIN Ying-ling1,XUE Xian-zi1,CHANG Ya-qing1
1.Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023, China;2.Xiangya Shool of Medicine Central South University,Changsha 410013,China
Abstract: The polysaccharides were extracted from intestines of sea cucumber Apostichopus japonicus by alkaline hydrolysis and enzymatic hydrolysis,and H22 tumor bearing mouse were established.The inhibiting effect of intestinal polysaccharide to H22 tumor were studied by celiac injection of polysaccharides at different doses(200,100 mg/(kg·d)and 50 mg/(kg·d)).The tumor inhibiting rates,thymus index,spleen index,IL-2,and TNF-α were determined 15 days after intestinal polysaccharide injection.The sea cucumber injected by the intestinal polysaccharides showed anti-tumor efficacy on H22 tumor bearing mice.There were significant higher tumor inhibition rates in the polysaccharide treatment groups than that in the control group(high dose group P＜0.01;middle,low dose groups P＜0.05).The spleen index was found to be increased with dose increase in the polysaccharide treatment groups compared with the control group,without significant difference.There was higher thymus index in the polysaccharide treatment groups than that in the tumor control group(P＜0.05).The maximal IL-2 content was observed in the high dose polysaccharide group,significant higher than that in the three control groups(P＜0.01). However,The IL-2 contents in the middle and low dose polysaccharide groups were not found significant difference with the tumor and polysaccharid control groups(P＞0.05).There were no statistical differences in TNF-α levels in all polysaccharide dose groups(P＞0.05).
2012, Vol. 27 
