WANG Ling1,2,ZHOU Yi-bing2,YANG Da-zuo2,YUAN Xiu-tang3
1.Fisheries College,Jimei University, Key Laboratory of Aquaculture Technology and Food Safe in Fujian Colleges, Xiamen 361021, China;2. Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023,China; 3. National Marine Environmental Monitoring Center, Dalian 116023, China
Abstract: Nitrogen utilization and nitrogen budget were studied in sluge wormPerinereisaibuhitensiswith body weight of (0.40±0.19)g(Group S), (1.00±0.22)g (Group M),and (2.00±0.50)g(Group L) collected from seaside of Zhuanghe, Dalian,Liaoning Province, reared in 10 cm thick sand tanks with circulating water, and fed flounder feed, at various temperature of 16,20 and 24 ℃. 1)The nitrogen consumption of the sluge worm was found to be ranged from 14.3 mg/(d·g)to 22.0 mg/(d·g), with average 17.0 mg/(d·g) at 20 ℃, higher than that at 16 ℃ and 24 ℃. 2)At the same water temperature, the nitrogen consumption was depressed with increase in body weight, from 10.5 mg/(d·g)to 22.0 mg/(d·g) in Group S, from 8.0 mg/(d·g)to 14.7 mg/(d·g) in Group M, and from 6.6 mg/(d·g)to 14.3 mg/(d·g) in Group L.The relationship between nitrogen consumption [CN, mg/(d·g)] and the body weight (W, dry body weight, g) was expressed asCN=aWb, with the maximumaof 10.618 at 20 ℃,and thebvalue from -0.2076.3 to -0.1911. The nitrogen consumption were significantly affected by temperature (F=79.125,P<0.001) and body weight (F=34.308,P<0.001).4)Under the experimental conditions, the average nitrogen budget was expressed as 100CN=88.99PN+1.58UN+9.43FN, with the average nitrogen retention of 11.8 mg/(d·g).