Abstract: Removal rates of total organic carbon (TOC), total ammonia nitrogen (TAN) and nitrite nitrogen were monitored in stimulant culture effluent of Pacific white leg shrimpLitopeanaeusvannameiin a circulating water operation device in which barnacle shells and polyethylene (PE) were used as biological filter materials and in which there was C∶N ratio of 5, 10, 20, and 30 to explore the effects of different filter materials and C∶N ratios on treatment of the effluent of Pacific white leg shrimp culture. The results showed that there were higher TAN and nitrite removal rates in the circulating water operation device with barnacle shells and at C∶N ratio of 5, 10 and 20 than those in the device with PE(P<0.05), without significant difference in TAN and nitrite removal rate in both treatment groups at C∶N ratio of 30(P<0.05). In the same filter material, however, there was significantly higher TAN removal rate, with TAN removal rate of over 98.0%, at C∶N ratio of 20 and 30 than that at C∶N ratio of 5 and 10 after 30 h(P<0.05). The removal rate of nitrite in barnacles shell and PE groups was shown to be significantly higher at C∶N ratio of 10, 20 and 30, with removal rate of over 93.79%, than that at C∶N ratio of 5 in the same filter material after 30 h(P<0.05). It is concluded that it is more appropriate to use barnacle shells as a filter material under C∶N=20∶1 for treatment of the culture effluent of Pacific white leg shrimp.