The toxicity of azadirachtin to survival,growth and reproduction of water fleas Daphnia magna
LIU Qing1,LI Yang1,HE Fang1,ZHANG Qing-jing2,NI Jian3,SHAO Sen-lin1
1.Key Laboratory of Hydrobiology in Liaoning Province,Dalian Ocean University,Dalian 116023,China;2.Beijing Fisheries Research Institute, Beijing 100068,China;3.Xinjiang Saihu Fishery Technology Development Co.,Ltd.,Bole 833400,China
Abstract: The toxic effects of 0.32%azadirachtin EC on survival,growth and reproduction of water fleas Daphnia magna were studied by acute and chronic toxicity tests.The acute toxicity test showed that azadirachtin as a highly toxic substance had the 24 h LC50of 39 μg/L,48 h LC50of 17 μg/L and the safety concentration of 1.12 μg/L for the water fleas.The Chronic toxicity test revealed that the water fleas showed decrease in intrinsic rate of population increase(rm),number of clutches per female,total offspring per female,generation time(T),net reproductive rate(R0),and survival time with the increasing azadirachtin concentration.There was no significant difference in the rmin the water fleas between the control group and low concentration groups(0.432,0.768 and 1.120 μg/L)(P＞0.05).The rmwas decreased from 0.347 6 d-1at insecticide concentration of 1.340 to 0.306 3 d-1at insecticide concentration of 2.400 μg/L.The minimal number of clutches per female(2.47 times)and total offspring per female(26.87 ind.),the minimal survival time(13.60 d),the minimal R0(24.696 1),and T(11.439 9 d) were observed at the maximal concentration of 2.400 μg/L.In conclusion,the findings show that high concentrations of azadirachtin leads to short survival time,and T,and to reduce in R0number of clutches per female and total offspring per female significantly.
刘青, 李扬, 何芳, 张清靖, 倪健, 邵森林. 印楝素对大型溞生存、生长和繁殖的毒性效应[J]. 大连海洋大学学报, 2013, 28(2): 121-126.
LIU Qing, LI Yang, HE Fang, ZHANG Qing-jing, NI Jian, SHAO Sen-lin. The toxicity of azadirachtin to survival,growth and reproduction of water fleas Daphnia magna. Journal of Dalian Ocean University, 2013, 28(2): 121-126.
2013, Vol. 28 
