Abstract: In this paper,turbot(Scophthalmus maximus)juveniles weighing(29.00±1.82)g were fed diets containing 7 Chinese herbal medicines(CHM)immunostimulants including Achyranthes,Isatis tinctoria L.,Glycyrrhiza glabra,Pericarpium citri reticulatae,Cortex cinnamomi,Fructus hordei germinatus,and Massa medicata fermentata,which were grinded and screened with 80 mesh,at a rate of 0,2.0%,4.0%and 6.0%for 4 weeks to evaluate the effect of CHM on non-specific immunity in turbot.The results showed that there was significant influence of CHM on the activities of lysozyme,anti-bacterial and anti-protease,complement C3 content,number of NBT-positive cells and the phagocytic activity of pronephros leukocytes.With the extension of feeding time,most of immunological factors were found to have a tendency of increase first and then decrease,the peak activity on the 2ndor 3rdweek.There were significantly higher lysozyme and anti-bacterial activities in the 4%group than those in the control group during the experiment(P＜0.05).The higher anti-protease activity was observed in 4%and 6% groups in the first two weeks.However,the anti-protease activity in 2%group was continued to increase in later two weeks,significantly higher than in other groups(P＜0.05).There was significantly higher complement C3 content in 4%group than that in the control group(P＜0.05).The juveniles fed the diet containing Chinese herbal medicines had significantly more NBT-positive cells than the fish in the control group did(P＜0.05),especially the fish fed the diets containing 4%and 6%Chinese herbal medicines.The phagocytic activity of pronephros leukocyte was found to be decreased first and increased later in 4%and 6%groups throughout the experiment,the peak at the 4th week,and significantly higher than that in 2%group and the control group(P＜0.05).Infection test showed that the survival rate in the groups was descendantly arranged as 4%grouP＞2%grouP＞6%grouP＞control group.It is concluded that addition of 2%CHM to the basal food can improve the non-specific immunity and disease resistance in turbot,especially in 4%group.
李华, 张太娥, 李强. 复方中草药对大菱鲆非特异性免疫力的影响[J]. 大连海洋大学学报, 2013, 28(2): 115-120.
LI Hua, ZHANG Tai-e, LI Qiang. Influence of compound Chinese herbal medicine on non-specific immunity of turbot Scophthalmus maximus. Journal of Dalian Ocean University, 2013, 28(2): 115-120.
2013, Vol. 28 
