Abstract: Techniques on artificial seeding of \%Undaria pinnatifida\% was studied indoors. \%Undaria pinnatifida\% cultivated in Dalian sea waters can release spores at water temperature of 14~21℃ and achieved a peak of over 60 million spores/cm\+2 at 16.0~18.5℃. Seed collecting through spores was conducted by using vinylon rope substrata, and the optimum concentration of spores was 60 individuals/visual field of microscope(×100),spores take 2.0 h or more to adhere firmly.Gametophytes grew well at 18~22℃, pass the summer at over 23℃ and the suitable light intensity should be 300 lx.The gametophytes matured at 22~20℃ and the rate of maturation was up to 80% or more under the light intensity of 2000 lx.The young sporophytes grew rapidly at 21~18℃,reaching 300μm or more in length after fertilizing 15 days at light intensity of 3000 lx.The young sporophytes should be moved out of nursery pool at 20℃,which can reach the standard of seed seperation after 30 days rearing in sea waters. The seedings of 1.0~2.0 cm in length were cultured in the sea waters in 30 days. Two million meter seed ropes with the seeding density of 20~200 strains/cm and the length of 1.0~2.0 cm were cultured from 1994 to 1997.
张泽宇, 曹淑清, 由学策, 范春江, 苏延明. 裙带菜室内人工育苗的研究[J]. 大连海洋大学学报, 1999, 14(2): 7-13.
Zhang Zeyu, Cao Shuqing, You Xuece, Fan Chunjiang, Su Yanming. Techniques on artificial seeding on \%Undaria Pinnatifida. Journal of Dalian Ocean University, 1999, 14(2): 7-13.