Abstract: The semen of pearl oyster Pinctada martensii was smeared and stained by two dyes Eosin Y and Crystal Violet for comparison of the staining effect and to evaluate the sperm quality in order to find out the suitable dying methods for the analysis of sperm morphology of pearl oyster.It was found that the sperm head was found to be dyed incompletely,and the sperm color was not improved with the extension of time,without obvious characteristics when the semen smear was stained with 0.8 mg/mL Eosin Y.Thus,this method is unfit for the sperm characteristic detection.After the sperm solution was mixed with 20 mg/mL Eosin Y,the sperm head was cardinal,and the tail plum,with clear parts of the sperm structure,obvious morphological characteristics and the very fair background. The obvious sperm characteristics including the dark violet sperm head and the light purple tail with the very fair background were observed in the semen stained with 5 mg/mL of Crystal Violet.So this method can be used to evaluate the sperm characteristics precisely.The main malformation was found in the pieces and tail of the sperm, few in the head according to the sperm characteristics shown by staining.The detection revealed that there were no significant differences in the evaluation of the normal sperm by the 5 mg/mL of Crystal Violet and by latter two methods(P＞0.05),so either could be used to evaluate the sperm quality.
宾承勇, 王梅芳, 豆伟, 余祥勇. 马氏珠母贝精子质量形态评定中不同染色法染色效果的比较[J]. 大连海洋大学学报, 2012, 27(1): 32-37.
BIN Cheng-yong, WANG Mei-fang, DOU Wei, YU Xiang-yong. Comparison of different staining methods for morphological evaluation of the sperm quality in pearl oyster Pinctada martensii. Journal of Dalian Ocean University, 2012, 27(1): 32-37.
2012, Vol. 27 
