Abstract: The chemiluminescence immunoassay(CLIA)method,luminol as CLIA substrate and H2O2as oxidant of luminal,was established for detection of organochlorine pesticides DDTs residues.The results showed that luminescence substrate solution had low and constant luminescence values in 5-7 minutes under uncatalyzed condition, and the peak of the luminescence substrate solution was observed in 10 minutes under catalyzed condition and then the luminescence values began to decrease.The standard curve was found to be linear from 0.05 ng/mL to 25 ng/mL,and linear equation was expressed as y=-63.806x+13426,R2=0.9945,with the minimal detection limit of 0.05 ng/mL,and the recovery varying from 91.4%to 107.8%.
2011, Vol. 26 
