Abstract: Accumulation of metsulfuron-methyl residues in Nile tilapia Oreochromis niloticus was studied under simulated pond conditions by14C isotopic tracer technique.The quantity of metsulfuron-methyl residues were found different in the fish tissues.Compared with other tissues of fish,the maximal amount of accumulated metsulfuronmethyl was observed in the flesh,even though the minimal concentration of metsulfuron-methyl residues in the same tissue.The dynamic of metsulfuron-methyl residues in the fish tissues demonstrated that the duration for reaching the balance between the accumulation and elimination was found different.The less required duration was found in the fish viscera and more required duration in the truck for the balance.The tendency of metsulfuronmethyl residues in fish viscera was expressed as elimination,whereas the fish head and truck showed accumulation at the initial stages of the experiment,and then it gradually changed to elimination.