Abstract: The minimum inhibitory concentrations and minimum bactericidal concentrations of 10 kinds of Chinese herbal medicines including Fructus Psoraleae,Cortex Cinnamomi,Rhizoma Coptidis,Galla Chinensis,Pericarpium Granati,Radix et Rhizoma Rhei,Radix Scutellariae,Radix Sophorae Flavescentis,Fructus Mume and Radix Pulsatillae extracted by decoction by boiled water,microwave by ethanol and microwave by boiled water to 7 marine pathogenic bacteria strains were determined.Also,susceptibility of the pathogenic bacteria to the combination of Chinese herbal medicines was carried out to evaluate antibacterial action of several Chinese herbal medicines to marine major pathogenic bacteria.It was found that there were best bacteriostatic and bactericidal effect of Galla Chinensis and Pericarpium Granat,followed by Rhizoma Coptidis,Radix Scutellariae and Fructus Mume,and then Radix et Rhizoma Rhei,Fructus Psoraleae,Cortex Cinnamomi,Radix Sophorae Flavescentis and Radix Pulsatillae. The MIC was found to be 0.78-1.56 mg/mL(decoction or microwave by water)and 0.39-1.56 mg/mL(microwave by ethanol)for Galla Chinensis,1.56-3.13 mg/mL(decoction by water),0.78-1.56 mg/mL(microwave by ethanol)and 0.78-3.13 mg/mL(microwave by water)for Pericarpium Granati.MBC was 3.13-6.25 mg/mL (decoction by water)and 1.56-3.13 mg/mL(microwave by ethanol or water)for Galla Chinensis,6.25-25 mg/ mL(decoction by water),3.13-6.25 mg/mL(microwave by ethanol or water)for Pericarpium Granati.Chinese herbal medichines extracted with different methods showed differently antibacterial effect,being arranged as microwave by ethanol≥microwave by water≥decoction by water.There were synergistic inhibition of combination of Galla Chinensis and Fructus Mume,Galla Chinensis and Radix Glycytthizae for Vibrio splendidus,Radix Scutellariae and Radix Glycytthizae for Edwardsiellosis tarda,Pericarpium Granat and Fructus Mume for Streptococcus iniae, Galla Chinensis and Pericarpium Granati,Galla Chinensis and Radix Scutellariae for Shewanella marisflavi.
2011, Vol. 26 
