Acute toxicity of phenol, copper, nitrite and total ammonia to juvenile Chinese sturgeon, Acipenser sinensis
DU Hao, WEI Qi-wei, LIU Jian-yi,YANG De-guo,CHENG Xi-hua, ZHU Yong-jiu, TANG Guo-pan
1. Key Lab of Freshwater Fish Germplasm Resources and Biotechnology, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China;2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 3. Fisheries College, Huazhong Agricultural University, Wuhan 430070, China
Abstract: Acute toxicities of phenol, Cu^2＋ , nitrite and total ammonia to juvenile Chinese sturgeon Acipenser sinensis fingerlings were determined in static bioassays. The juveniles with （2.36±0.10 ）cm in total length and average 0.07 g in body weight were 8 - 12 day old just before the yolk would be completely exhausted. It was found that the 24, 48, 72 h and 96 h median lethal concentration （LCso） values （95% confidence interval ） of the phenol to the juveniles were 97 （87 -109）,83 （73 -95 ）, 75 （69-83 ）mg/L and71 （64-80 ）mg/L, respectively. The 24, 48, 72 h and 96 h LCso values （95% confidence interval ） of Cu^2＋ to the juveniles were 0.31 （0.29 -0.34）, 0.20 （0.15 -0.27 ）, 0.09 （0.08 -0.12 ）mg/L and 0.07 （0.06 -0.10 ）mg/L, respectively. The24 , 48, 72 h and 96 h LCso values （95% confidence interval ） of nitrite - N to the juveniles were 7.05 （6.22 - 8.03 ）, 4.07 （1.51 -7.88 ）, 3.10 （2.07 -4.34 ）mg/L and 2.57 （2.31 -2.85 ）mg/L, respectively. The 24, 48, 72 h and 96h LCso values （95% confidence interval ） of unionized ammonia - N to the juveniles were 0.67 （0.64 - 1.07）, 0.52 （0.44-0.67）, 0.43 （0.40-0.54） mg/L and 0.39 （0.30-0.45）mg/L, respectively. The juveniles had higher heart beating rates （ 119 ＋ 10 beat/min, n = 10） in 126 mg/L phenol than those in the control group （106±5 beat/min, n = 10） （ P 〈 0.05）. There was no significant difference in heart raites in 0.4 mg/L Cu^2＋ and in the control group（ P 〉 0.05）. The juveniles had significantly higher heart beating rates in 20.30 mg/L nitrite - N （ 112 ±4 beat/min, n = 10） and 29.20 mg/L total ammonia - N （ 112±7 beat/min, n = 10） than in the control group （ P 〈 0.05 ）.
2007, Vol. 22 
