Abstract: Nine diets formulated by L9 (3^4) orthognnal design were fed to Schizothorax prenarai juveniles weighing (2.14 ±0.31) g to evaluate the requirements for protein, fat, mineral and total energy. The results showed that the influence of nutrients on growth and protein efficiency ranged in a descendant order as: protein 〉 total energy 〉 fat 〉 mineral. The optimum requirement for dietary protein, energy, fat, mineral and energy -protein ratio (E/P)was40.06%, 13.09 kJ/g, 6.02%, 4% and 32.68 kJ/g in the diets of Schizothorax prenanti, respectively. There was a significant effect of dietary protein level on growth and body composition in Schizothorax prenanti (P〈 0.05).