Abstract: In this study, the extraction of phospholipids from skipjack brain was studied by ultrasonic method, and optimized by a single factor experiment and orthogonal experiment. The optimal conditions or extraction yield of phospholipids were obtained as the following conditions of the ratio of hexane and ethanol=2∶1, 40 min,35 ℃, the ratio of material to solvent=1∶9, and ultrasonic method for twice, with the phospholipids extraction efficiency of 87.1% relative to the chloroform-methanol method. The thin-layer chromatography(TLC)revealed that the phospholipids contained phosphatidylethanolamine(PE), phosphatidylcholine (PC),and sphingomyelin (SM). The gas chromatography analysis showed that there were high unsaturated fatty acids including 21.1% of DHA and 2.4% EPA in the phospholipid fatty acids of skipjack brain. The findings indicate that it is feasible to extract phospholipids from the fish brain by ultrasonic method, and that there is rich in unsaturated fatty acids in the phospholipids of the skipjack brain，showing some potential for development in food processing.
2014, Vol. 29 
