Abstract: A chloramphenicol(CAP) molecularly imprinted film was prepared on the surface of MaxiSorp polystyrene 96-well plates using chloramphenicol as molecular template, tetrahydrofuran as solvent and azobisisobutyronitrile as initiator with a UV lamp，and the preparation conditions were optimized by a series of experiments. The results showed that the imprinted film exhibited good adsorption at ratio of the template, functional monomer(EDAEM) and cross-linker(EGDMA) = 1∶4∶1, the template dosage of 0.25 mmol and the solvent of 5 mL. The adsorption equilibrium was found within 50 min and the adsorption of 1.51 μg/well was observed at initial chloramphenicol concentration of 120 mg/L. The chloramphenicol adsorption of 0.83 μg/well on the imprinted film was observed at the initial CAP, TAP and FF concentration of 50 mg/L. The florfenicol adsorption of only 0.40/well, and thiamphenicol adsorption of 0.49 μg/well were found at that CAP, TAP and FF concentration, indicating that the imprinted film can be used as an artificial antibody in chemiluminescence immunoassay based on the principle of direct competition for detection of chloramphenicol.
2014, Vol. 29 
