长牡蛎牛磺酸转运蛋白CgTauT基因的结构和表达特征

长牡蛎牛磺酸转运蛋白CgTauT基因的结构和表达特征

姜春雨, 孔宁, 孙士晴, 李想, 罗聪, 乔鑫, 王玲玲, 宋林生

大连海洋大学学报 ›› 2025, Vol. 40 ›› Issue (1) : 25-33.

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大连海洋大学学报 ›› 2025, Vol. 40 ›› Issue (1) : 25-33. DOI: 10.16535/j.cnki.dlhyxb.2024-125

长牡蛎牛磺酸转运蛋白CgTauT基因的结构和表达特征

姜春雨，孔宁*，孙士晴，李想，罗聪，乔鑫，王玲玲，宋林生*

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Structure and expression characteristics of taurine transporter CgTauT genes in Crassostrea gigas

JIANG Chunyu，KONG Ning*，SUN Shiqing，LI Xiang，LUO Cong，QIAO Xin，WANG Lingling，SONG Linsheng*

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摘要

为探究长牡蛎(Crassostrea gigas)(湿质量为155.0 g±5.0 g)牛磺酸转运蛋白( taurine transporter，TauT)基因家族成员组成及其表达特征，采用全基因组筛查策略对长牡蛎牛磺酸转运蛋白基因进行鉴定，并利用qRT-PCR对其组织分布模式和牛磺酸处理下的表达特征进行分析。结果表明：长牡蛎基因组中存在4个CgTauT基因，均包含1个SNF结构域和12个跨膜结构域；4个CgTauT基因在长牡蛎各组织中呈组成性表达，其中CgTauT1在闭壳肌中表达量最高，CgTauT2、CgTauT3和CgTauT4在外套膜中表达量最高，不同组织中CgTauT基因表达量与牛磺酸含量无显著相关性；牛磺酸浸泡处理下4个CgTauT基因在外套膜中呈现不同的时序表达模式，其中CgTauT1的表达量在处理3 h后率先升高，CgTauT3的表达量随处理时间延长呈逐渐升高趋势，CgTauT4的表达量随处理时间延长呈先升高后降低的趋势。本研究结果为深入探究CgTauT的生理功能和长牡蛎的分子机制提供了科学参考。

Abstract

To investigate the composition and expression characteristics of the taurine transporter (CgTauT) gene family in Crassostrea gigas,a genome-wide screening strategy was used to identify taurine transporter genes,and the qRT-PCR was used to analyse their tissue distribution pattern and to expression characteristics after taurine treatment.The results revealed that C. gigas contains four CgTauT genes,with each containing an SNF structural domain and 12 transmembrane domains.The four CgTauT genes were constitutively expressed in the various tissues of C. gigas,with CgTauT1 showing the highest expression in the adductor muscle,and CgTauT2,CgTauT3 and CgTauT4 showing the highest expression in the mantle.There were no significant correlations between the expression levels of the four CgTauT genes and the taurine contents of the tissues.Under taurine immersion treatment,the four CgTauT genes exhibited different temporal expression profiles in the mantle of C. gigas.The expression level of CgTauT1 increased after three hours of treatment.With prolonged taurine treatment,the mRNA expression level of CgTauT3 increased gradually,while that of CgTauT4 first increased and then decreased.These findings provide a theoretical foundation for further exploration of the physiological functions of CgTauTs and the molecular mechanisms underlying taurine accumulation in C. gigas.

关键词

长牡蛎 / 牛磺酸转运蛋白 / 组织分布 / 基因家族 / 表达特征

Key words

Crassostrea gigas / taurine transporter / tissue distribution / gene family / expression profile

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姜春雨, 孔宁, 孙士晴, 李想, 罗聪, 乔鑫, 王玲玲, 宋林生. 长牡蛎牛磺酸转运蛋白CgTauT基因的结构和表达特征[J]. 大连海洋大学学报, 2025, 40(1): 25-33 https://doi.org/10.16535/j.cnki.dlhyxb.2024-125

JIANG Chunyu, KONG Ning, SUN Shiqing, LI Xiang, LUO Cong, QIAO Xin, WANG Lingling, SONG Linsheng. Structure and expression characteristics of taurine transporter CgTauT genes in Crassostrea gigas[J]. Journal of Dalian Fisheries University, 2025, 40(1): 25-33 https://doi.org/10.16535/j.cnki.dlhyxb.2024-125

中图分类号： S 917.4

基金

大连市高层次人才创新支持计划(2022RG14)

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