为探究青海湖裸鲤(Gymnocypris przewalskii)(体质量为126.67 g±8.88 g)如何通过糖代谢响应碱度变化,试验设置了CK、J25%、J50%、J75%和J100%共5个不同碱度处理组,测定了不同碱度下糖原(Glycogen)、葡萄糖(GLU)、丙酮酸(PA)、己糖激酶(HK)、丙酮酸激酶(PK)、磷酸烯醇式丙酮酸羧激酶(PEPCK)、6-磷酸葡萄糖脱氢酶(G6PD)和果糖-1,6-二磷酸醛缩酶(FBA)等糖代谢重要指标。结果表明:随着碱度的增高,青海湖裸鲤肝糖原、肌糖原及鳃、肾、肝中的GLU先呈升高再降低的趋势,肌中GLU逐渐降低;鳃中PA呈先升高再降低的趋势,肾、肝和肌中PA呈先降低再升高的趋势;鳃中HK、PK、PEPCK和G6PD活性呈上升趋势,肾、肝中FBA活性逐渐上升;热图分析显示,青海湖裸鲤鳃、肾和肝中糖代谢过程均被碱度不同程度地促进,肌中糖代谢则被碱度抑制;果糖-1,6-二磷酸醛缩酶C(fba-c)在鳃、肾、肝等组织中普遍表达,随碱度的升高,鳃、肾中fba-c的表达呈先上升再降低的趋势,其中J75%组最高。研究表明,青海湖裸鲤鳃、肾、肝可通过糖代谢的增强响应碱度变化,肌肉则通过抑制糖代谢应对碱度变化,从而使青海湖裸鲤快速适应不同碱度。
Abstract
To inquire mechanism of Gymnocypris przewalskii adaptation to alkalinity changes through glucose metabolism,G. przewalskii with body weights of 126.67 g±8.88 g were reared in an 85 cm×56 cm×62 cm tank containing either freshwater (control group) or four experimental alkalinities of 25%,50%,75% and 100%,prepared using NaHCO3 and Na2CO3 dissolved in tap water (NaHCO3∶Na2CO3 = 9∶1).The important indicators of G. przewalskii glucose metabolism were determined,including glycogen content,glucose (GLU) content,and the activities of pyruvate (PA),hexose kinase (HK),pyruvate kinase (PK),phosphoenolpyruvate (PK),phosphoenolpyruvate carboxylase (PEPCK),glucose 6 phosphate dehydrogenase (G6PD),and fructose 1,6 bisphosphate aldolase (FBA).The results showed that,with increasing alkalinity,the hepatic glycogen,muscle glycogen,and GLU content in the gills and kidneys increased and then decreased,and the GLU in the muscle gradually decreased.As the alkalinity increased,PA in the gills increased and then decreased,and PA in the kidneys,liver,and muscle decreased and then increased.The activities of HK,PK,PEPCK and G6PD in the gills showed an increasing trend with increasing alkalinity,and the activities of FBA in the kidneys and liver gradually rose.A heatmap showed that glucose metabolism processes in the gills,kidneys,and liver were promoted by increasing alkalinity to some extent,while glucose metabolism in the muscle was inhibited as alkalinity increased.As alkalinity increased,fba-c was commonly expressed in the gills,kidneys,intestine,and liver,and the expression level of fba-c in the gills and kidneys increased and then decreased.The maximum expression levels were observed in the 75% alkalinity group.In conclusion,the gills,kidneys,and liver responded to alkalinity changes by increasing glucose metabolism,while muscle responded to alkalinity changes by inhibiting glucose metabolism,leading to rapid adaptation to different alkalinity levels in G. przewalskii.
关键词
青海湖裸鲤 /
碱胁迫 /
糖代谢 /
应答
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Key words
Gymnocypris przewalskii /
alkalinity stress /
glucose metabolism /
response
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中图分类号:
S 917.4
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