克氏原螯虾3群体双列杂交群体耐高温性能比较

吴玮杰, 徐啟洲, 万通, 贾亮, 程熙, 李典中, 白志毅

大连海洋大学学报 ›› 2024, Vol. 39 ›› Issue (4) : 606-612.

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大连海洋大学学报 ›› 2024, Vol. 39 ›› Issue (4) : 606-612. DOI: 10.16535/j.cnki.dlhyxb.2023-281

克氏原螯虾3群体双列杂交群体耐高温性能比较

  • 吴玮杰,徐啟洲,万通,贾亮,程熙,李典中,白志毅*
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Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish (Procambarus clarkii)

  • WU Weijie,XU Qizhou,WAN Tong,JIA Liang,CHENG Xi,LI Dianzhong,BAI Zhiyi*
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摘要

为构建耐高温性状的克氏原螯虾(Procambarus clarkii)杂交群体,以芜湖、建湖、滆湖3个克氏原螯虾群体完全双列杂交的9个F1群体为亲本,通过自交获得F2群体[滆湖♀×建湖♂(GJ)、建湖♀×滆湖♂(JG)、滆湖♀×芜湖♂(GW)、芜湖♀×滆湖♂(WG)、建湖♀×芜湖♂(JW)、芜湖♀×建湖♂(WJ)、滆湖♀×滆湖♂(GG)、建湖♀×建湖♂(JJ)、芜湖♀×芜湖♂(WW)],采用耐热性(upper thermal tolerance,UTT)、心率的阿伦尼乌斯拐点温度(arrhenius break temperatures,ABT)作为评定指标,并结合临界温度法(critical thermal methodology,CTM)、半致死时间(half leathaltime,LT50)等方法,比较了9个F2群体对高温的耐受性,并进一步分析了克氏原螯虾UTT值、ABT值与体质量等性状的相关性。结果表明:各群体UTT值排序为GW>GJ>WJ>JG>GG>WW>WG>JW>JJ,且GW群体UTT值显著高于其余群体(P<0.05);各群体ABT排序为GW>WJ>WW>WG>JW>JJ>JG>GJ>GG,且GW群体ABT显著高于其余群体(P<0.05);各群体CTMax排序为GW>WJ>GJ>GG>WW>JG>JW>WG>JJ,且GW、WJ、GJ群体显著高于其余群体(P<0.05);各群体LT50排序为GW>GJ>WJ>JG>WW>GG>JW=JJ>WG;不同规格克氏原螯虾个体之间耐热性(UTT)存在显著性差异(P<0.05),即克氏原螯虾虾苗耐热性显著高于初达上市规格的商品虾(P<0.05);克氏原螯虾热胁迫前后心率均与体质量呈负相关,相关系数分别为-0.21和-0.15(P<0.05)。研究表明,滆湖♀×芜湖♂(GW)群体4个耐高温指标均优于其他群体,与UTT等指标相比,采用ABT指标测定对个体损害小,更适用于良种选育。

Abstract

In order to establish a hybrid population of red swamp crayfish (Procambarus clarkii) with high temperature tolerance, nine F1 populations of complete diallel hybridization from three populations of red swamp crayfish,Wuhu, Jianhu, and Gehu, were used as broodstocks to obtain F2 populations through self pollination [Gehu♀×Jianhu♂(GJ), Jianhu♀×Gehu♂(JG), Gehu♀×Wuhu♂(GW), Wuhu♀×Gehu♂(WG), Jianhu♀×Wuhu♂(JW), Wuhu♀×Jianhu♂(WJ), Gehu♀×Gehu♂(GG), Jianhu♀×Jianhu♂(JJ), and Wuhu♀×Wuhu♂(WW)]exposed to the water temperature from 30 ℃ to 35 ℃ at raising rate of 1 ℃/2 h,and at raising rate of 0.5 ℃/4 h until the crayfish death. The tolerance of 9 F2 populations to high temperature was compared using evaluation indicators of upper thermal tolerance (UTT) and Arrhenius Break Temperatures (ABT) of heart rate, combined with Critical Thermal Methodology (CTM), median lethal Time (LT50) and other methods, and the correlation was analyzed between ABT values and traits including body weight. The results showed that the descending order of the UTT values in each group were ranked as GW>GJ>WJ>JG>GG>WW>WG>JW>JJ, with significantly higher in the GW group than that in the other groups (P<0.05). The ABT was expressed as GW>WJ>WW>WG>JW>JJ>JG>GJ>GG in each group, significantly higher in the GW group than that in the other groups (P<0.05). The CTMax was found to be GW>WJ>GJ>GG>WW>JG>JW>WG>JJ in different groups, significantly higher in the GW, WJ, and GJ groups than that in the other groups (P<0.05). The LT50 was described as GW>GJ>WJ>JG>WW>GG>JW=JJ>WG in the red swamp crayfish in each group. There was significant difference in heat tolerance (UTT) among individuals in the crayfish with different developmental stages(P<0.05), significantly higher heat tolerance in crayfish juveniles than that in remarkable crayfish just reached the market specifications (P<0.05). Before and after heat stress, there was a negative correlation between heart rate and body weight in crayfish, with correlation coefficients of -0.21 and -0.15, respectively (P<0.05). The (GW) population had superior to other populations in four high temperature tolerance indicators. Compared with UTT and other indicators, using ABT indicators to measure was less harmful to individuals and more suitable for breeding high-quality varieties.

关键词

克氏原螯虾 / 耐高温性能 / 耐热性 / 心率拐点温度 / 群体

Key words

Procambarus clarkii / high temperature resistance performance / upper thermal tolerance (UTT) / arrhenius break temperatures(ABT) / population

引用本文

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吴玮杰, 徐啟洲, 万通, 贾亮, 程熙, 李典中, 白志毅. 克氏原螯虾3群体双列杂交群体耐高温性能比较[J]. 大连海洋大学学报, 2024, 39(4): 606-612 https://doi.org/10.16535/j.cnki.dlhyxb.2023-281
WU Weijie, XU Qizhou, WAN Tong, JIA Liang, CHENG Xi, LI Dianzhong, BAI Zhiyi. Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish (Procambarus clarkii)[J]. Journal of Dalian Fisheries University, 2024, 39(4): 606-612 https://doi.org/10.16535/j.cnki.dlhyxb.2023-281
中图分类号: S 966.12   

基金

上海市科技创新行动计划项目(23N11900300);安徽省水产种业振兴项目(2022AHNYNC081FW)

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