1.Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries,Shanghai Ocean University,Shanghai 201306,China;2.Shanghai Jiading District Fisheries Technology Promotion Station,Shanghai 201800,China;3.Wuhu Shengdian Leisure Ecological Park Company,Wuhu 241212,China;4.Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals,Shanghai Ocean University,Shanghai 201306,China
In order to establish a hybrid population of red swamp crayfish (Procambarusclarkii) with high temperature tolerance, nine F1 populations of complete diallel hybridization from three populations of red swamp crayfish,Wuhu, Jianhu, and Gehu, were used as broodstocks to obtain F2 populations through self pollination [Gehu♀×Jianhu♂(GJ), Jianhu♀×Gehu♂(JG), Gehu♀×Wuhu♂(GW), Wuhu♀×Gehu♂(WG), Jianhu♀×Wuhu♂(JW), Wuhu♀×Jianhu♂(WJ), Gehu♀×Gehu♂(GG), Jianhu♀×Jianhu♂(JJ), and Wuhu♀×Wuhu♂(WW)]exposed to the water temperature from 30 ℃ to 35 ℃ at raising rate of 1 ℃/2 h,and at raising rate of 0.5 ℃/4 h until the crayfish death. The tolerance of 9 F2 populations to high temperature was compared using evaluation indicators of upper thermal tolerance (UTT) and Arrhenius Break Temperatures (ABT) of heart rate, combined with Critical Thermal Methodology (CTM), median lethal Time (LT50) and other methods, and the correlation was analyzed between ABT values and traits including body weight. The results showed that the descending order of the UTT values in each group were ranked as GW>GJ>WJ>JG>GG>WW>WG>JW>JJ, with significantly higher in the GW group than that in the other groups (P<0.05). The ABT was expressed as GW>WJ>WW>WG>JW>JJ>JG>GJ>GG in each group, significantly higher in the GW group than that in the other groups (P<0.05). The CTMax was found to be GW>WJ>GJ>GG>WW>JG>JW>WG>JJ in different groups, significantly higher in the GW, WJ, and GJ groups than that in the other groups (P<0.05). The LT50 was described as GW>GJ>WJ>JG>WW>GG>JW=JJ>WG in the red swamp crayfish in each group. There was significant difference in heat tolerance (UTT) among individuals in the crayfish with different developmental stages(P<0.05), significantly higher heat tolerance in crayfish juveniles than that in remarkable crayfish just reached the market specifications (P<0.05). Before and after heat stress, there was a negative correlation between heart rate and body weight in crayfish, with correlation coefficients of -0.21 and -0.15, respectively (P<0.05). The (GW) population had superior to other populations in four high temperature tolerance indicators. Compared with UTT and other indicators, using ABT indicators to measure was less harmful to individuals and more suitable for breeding high-quality varieties.
WU Weijie, XU Qizhou, WAN Tong, JIA Liang, CHENG Xi, LI Dianzhong, BAI Zhiyi.
Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish (Procambarus clarkii)[J]. Journal of Dalian Fisheries University, 2024, 39(4): 606-612 https://doi.org/10.16535/j.cnki.dlhyxb.2023-281
中图分类号:
S 966.12
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