Effects of air exposure and recovery on antioxidant capacity, apoptosis and immune related gene expression in coelomocytes of sea urchin (Strongylocentrotus intermedius)
LIN Weigang，SUN Qi，HAI Hangyu，LIU Yating，ZHAO Peng，CHANG Yaqing，MA Deyou*
1.Key Laboratory of Mariculture &Stock Enhancement in North China’s Sea，Ministry of Agriculture and Rural Affairs，Dalian Ocean University，Dalian 116023，China；2.Shanghai Fisheries Research Institute，Shanghai 200433，China；3.College of Marine Life Sciences，Ocean University of China，Qingdao 266000，China
Abstract: To evaluate the effect of air exposure transportation on the survival of sea urchin (Strongylocentrotusintermedius), the total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were determined during 12, 24 and 36 h air exposure stress and subsequent 12 h recovery, and apoptosis was detected by flow cytometry, and the expression levels of lysozyme gene LYZ, TLR1, Caspase-3 and Caspase-9 in coelomocytes were investigated using real-time quantitative PCR. The main results showed that the T-SOD activity, MDA content, and T-AOC level generally increased first and then decreased during exposure to air, with the maximum values observed at 12 h air exposure stress and no significant differences between control and 12 recovery (P>0.05). Air exposure had significant effects on the expression of immune related genes LYZ and TLR1, reaching the maximum values at 24 and 36 h of air exposure, which were significantly higher than those in other stress groups (P<0.05). Their expression levels after 12 h recovery following exposure times 36 h were also significantly different from those in controls (P<0.05). It was identified that the change trend of apoptosis rates was similar to that of the T-AOC levels, reaching the maximum value at 12 h air exposure. However, the expression levels of Caspase-3 and Caspase-9 genes presented significant differences between control and time intervals (P<0.05), with the maximum values observed at 24 h and 36 h of air exposure, respectively, suggesting response of the two important apoptosis-related Caspases had time cumulative effect. These findings indicated that sea urchins S.intermedius have certain ability on air exposure tolerance, using antioxidant enzyme system to conserve the dynamic balance of reactive oxygen species (ROS). Innate immune molecules such as lysozyme and TLR1, as well as Caspase dependent apoptosis, play important roles in maintaining homeostasis in response to air exposure stress.
林威港, 孙琦, 海航瑜, 刘亚婷, 赵鹏, 常亚青, 马得友. 干露及恢复对中间球海胆抗氧化能力、细胞凋亡和免疫相关基因表达的影响[J]. 大连海洋大学学报, 2024, 39(1): 66-73.
LIN Weigang, SUN Qi, HAI Hangyu, LIU Yating, ZHAO Peng, CHANG Yaqing, MA Deyou. Effects of air exposure and recovery on antioxidant capacity, apoptosis and immune related gene expression in coelomocytes of sea urchin (Strongylocentrotus intermedius). Journal of Dalian Ocean University, 2024, 39(1): 66-73.
2024, Vol. 39 
