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大连海洋大学学报  2023, Vol. 38 Issue (5): 885-892    DOI: 10.16535/j.cnki.dlhyxb.2023-021
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海鲈混菌发酵工艺优化及其品质分析
聂诗,吴燕燕,赵永强,王悦齐*,李来好*
1.上海海洋大学 食品学院,上海 201306;2.中国水产科学研究院南海水产研究所 农业农村部水产品加工重点实验室,广东 广州 510300
Optimization and quality analysis of mixed bacterial fermentation in sea bass
NIE Shi,WU Yanyan,ZHAO Yongqiang,WANG Yueqi*,LI Laihao*
1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;2.Key Laboratory of Aquatic Products Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China
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摘要 为改善发酵海鲈(Lateolabrax japonicas)的风味和口感,以植物乳杆菌(Lactobacillus plantarum)和戊糖片球菌(Pediococcus pentosaceus)作为外加复合菌株发酵剂,以感官综合评分为评价指标,采用单因素试验与响应面法对海鲈混菌发酵工艺条件进行优化,并进一步探究了接菌发酵对海鲈品质的影响。结果表明:海鲈的最优发酵条件为戊糖片球菌与植物乳杆菌体积比为2∶1、接种菌量为1.0%、发酵时间为30 h和发酵盐添加量为3%;在此工艺条件下,与自然发酵海鲈相比,接菌发酵海鲈的a*值由-1.89增加至-0.94,b*值由1.71下降至1.48,发酵海鲈色泽得到改善;硬度、咀嚼性和胶着性分别由40.17 g、0.44 mJ、33.27 g增加至223.33 g、3.29 mJ、219.30 g,发酵海鲈质地得到明显改善;接菌发酵海鲈的总酸和氨基态氮含量分别提升了23.13%和40.07%。研究表明,混菌发酵有利于提升海鲈的品质和口感。
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聂诗,吴燕燕,赵永强,王悦齐,李来好
关键词:  海鲈  混菌发酵  发酵工艺优化  品质分析    
Abstract: In order to improve the flavor and texture of fermented sea bass, Lactobacillus plantarum and Pediococcus pentosaceus were used as additional composite strain fermenters, and sensory composite scores were used as evaluation indices to optimize the process conditions of mixed-bacteria fermentation of sea bass by using a single-factor test with the response surface method, and the effects of the inoculated fermentation on the quality of sea bass were further explored. The results showed that the optimal fermentation for sea bass were under conditions of 2∶1 volume ratio of P.pentosaceus to L.plantarum, 1.0% inoculum, 30 h fermentation and 3% fermented salt addition amount. Under this process condition, the a* value of inoculated fermented sea bass was increased from -1.89 to -0.94, b* decreased from 1.71 to 1.48, and improved color of fermented sea bass with natural fermented sea bass. The fermented sea bass had hardness increase from 40.17 g to 223.33 g, chewiness from 0.44 mJ to 3.29 mJ and gelatinousness from 33.27 g to 219.30 g, with significantly improved texture. The total acid mass fraction and amino nitrogen mass fraction were increased by 23.13% and 40.07% in the fermented sea bass with inoculated bacteria, respectively. Overall, the mixed bacterial fermentation of sea bass was beneficial to improve the quality and taste of sea bass.
Key words:  Lateolabrax japonicas    mixed bacterial fermentation    fermentation process optimization    quality analysis
               出版日期:  2023-11-08      发布日期:  2023-11-08      期的出版日期:  2023-11-08
中图分类号:  S 986.1  
基金资助: 国家自然科学基金(32001733);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-47);广东省基础与应用基础研究基金(2021A1515010833)
引用本文:    
聂诗, 吴燕燕, 赵永强, 王悦齐, 李来好. 海鲈混菌发酵工艺优化及其品质分析[J]. 大连海洋大学学报, 2023, 38(5): 885-892.
NIE Shi, WU Yanyan, ZHAO Yongqiang, WANG Yueqi, LI Laihao. Optimization and quality analysis of mixed bacterial fermentation in sea bass. Journal of Dalian Ocean University, 2023, 38(5): 885-892.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.16535/j.cnki.dlhyxb.2023-021  或          https://xuebao.dlou.edu.cn/CN/Y2023/V38/I5/885
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