1.College of Fisheries and Life Science，Shanghai Ocean University，Shanghai 201306，China；2.Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation，Ministry of Agriculture and Rural Affairs，Pearl River Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510380，China；3.College of Animal Science and Technology，Zhongkai University of Agriculture and Engineering，Guangzhou 510225，China
Abstract: In order to probe selective breeding effect of Chinese pond turtle (Mauremysreevesii), the genetic diversity and genetic structure were analyzed in five selective breeding generations of the wild and protospecies of Chinese pond turtle, including basic group F0 generation, and F1-F4 generation groups by polymorphic microsatellite (SSR) markers. The results showed that 103 alleles were detected in the 12 selected microsatellite loci, with the average allele number (Ha) of 8.58. The expected heterozygosity (He) was varied from 0.173 to 0.919, with mean of 0.581. The observed heterozygosity (Ho) was found to be 0.054-0.862, with an average of 0.479. Polymorphic information content (PIC) was changed from 0.165 to 0.914, with average of 0.531. There were 11 moderately or highly polymorphic loci in the 12 microsatellite loci, indicating that the microsatellite loci selected in this study can be used as a good evaluation tool for the analysis of genetic diversity and genetic structure of selective breeding generations of Chinese pond turtle. The (Ho) of the five selective breeding generations was 0.491, 0.494, 0.497, 0.458 and 0.453, respectively. The genetic differentiation coefficient (Fst) and AMOVA analysis revealed that the (Fst) values between the five breeding generations were 0.004-0.012, less than 0.05. The findings indicated that the genetic diversity of the selective breeding generation of Chinese pond turtle was decreased, with less degree of genetic differentiation between five generations. Therefore, the selective breeding population of the Chinese pond turtle still has the potential further breeding.
徐昊旸, 杨雪莹, 倪未, 刘芳, 陈海港, 朱新平, 刘晓莉. 乌龟选育世代遗传多样性及遗传结构的微卫星分析[J]. 大连海洋大学学报, 2023, 38(5): 812-818.
XU Haoyang, YANG Xueying, NI Wei, LIU Fang, CHEN Haigang, ZHU Xinping, LIU Xiaoli. Genetic diversity and genetic structure of selected generations of Chinese pond turtle (Mauremys reevesii) based on microsatellite DNA. Journal of Dalian Ocean University, 2023, 38(5): 812-818.
2023, Vol. 38 
