Application of single-virus tracking technology on study of virus infection in live cells:a review
QIN Qiwei
College of Marine Sciences, University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation; Guangdong Aquatic Animal Immunity and Healthy Aquaculture Engineering Technology Research Center, South China Agricultural University, Guangzhou 510642, China
Abstract: Viruses are extremely simple and tiny organisms that only replicate in hosts. However, they are one of the most important pathogens to humans and other animals, and cause serious threat to human health, biodiversity, economic development and social stability. Viral infections in host are complex and dynamic, and can hardly be tracked in real time by conventional methods, such as biochemical and molecular technology, and electronic microscopy. Single-virus tracking(SVT) technology offers a powerful tool to track the life cycles of viruses in living cells in real time, thus revealing a comprehensive and detailed processes of virus infection. In this article, we describe the SVT technology and its application in studies on virus infection mechanisms, especially on aquatic animal virology, and finally discuss the current problems and future prospects of SVT, such as development of strategies for labelling virus, protein, nucleic acid and cells, exploitation of imaging technique, and optimizing of algorithms for SVT, thus enabling the study of animal virus infection mechanism to be more precise and systematic. It is expected to provide theoretical guidance and technical support for preventing and controlling the outbreaks and prevalence of viral diseases in aquatic animals.
秦启伟. 单病毒示踪技术在病毒侵染机制及水生动物病毒研究中的应用进展[J]. 大连海洋大学学报, 2021, 36(6): 899-909.
QIN Qiwei. Application of single-virus tracking technology on study of virus infection in live cells:a review. Journal of Dalian Ocean University, 2021, 36(6): 899-909.
2021, Vol. 36 
