College of Animal Science and Technology,Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production &Construction,State Kay Laboratory Breeding Base for The Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Corps and The Ministry of Science and Technology,Tarim University,Aral 843300,China
Abstract: Broodstock Schizothoraxeurystomus collected from Kizil River Basin in Xinjiang Uygur Autonomous Region was induced to spawn by hormone injection of pituitary gland (PG),human chorionic gonadotropin (HCG) and luteinizing hormone-releasing hormone analogs (LHRH-A2)at various doses and artificial hatchery methods at water temperature from 10 ℃ to 20 ℃ in a 1 ℃ interval in 2019 to accomplish farming and stock enhancement of S.eurystomus.The results showed that success rate and hatching rate were significantly varied with the doses of hormones and water temperature,with the optimal hormone combinations of PG at 10.27 mg/kg,HCG at 300.00 IU/kg and LHRH-A2 of 19.41 μg/kg.In April and May 2019,the fertilized eggs of S.eurystomus were obtained by artificial propagation,and the development of embryo and larva was observed under artificial incubation conditions,with the optimum incubation water temperature of 16.0-17.0 ℃.S.eurystomus had egg diameters of (2.50±0.24)mm,and reached (3.43±0.11)mm after water swelling;under the condition of water temperature (16.19±0.08)℃,the fertilized eggs were hatched in 145 h and 48 min,with the total accumulated temperature of 2 364.80 ℃·h.The embryonic development process experienced seven stages:embryo disc,cleavage,blastula,gastrula,neuroembryo,organogenesis and hatching.The newly hatched larvae had total length of (8.03±0.15) mm and the yolk sac total length of (20.17±0.96)mm,with the completely exhausted yolk sac at (17.88±0.44)℃ for 480 h.The findings further enrich the early life history data of S.eurystomus and then provide essential data including water temperature regulation,and choice of hormone and dose for resources protection,and large scale artificial propagation of S.eurystomus.
2022, Vol. 37 
