Abstract: In this paper, bacteria were isolated and cultured from viscera and digestive tract of Canadian rock scallop Crassadoma gigantea and the first generation of congener rock scallop from artificial breeding in China by the traditional method, and bacterial community diversity was analyzed by 16 S rRNA identification, and total DNA using Ion S5TMXL high throughput sequencing platform. The results showed that 83 strains of culturable strains were found by the traditional method, and 31 strains of representative strains were sequenced and compared,including Psychrobacter, Planococcus, Vibrio,Pseudomonas, Pseudoalteromonas, Bacillus, Kocuria and Paracoccus. The high throughput sequencing indicated that the effective sequence community structure of the 4 samples was divided into 9 phyla: Proteobacteria, Deinococcus-Thermus, Bacteroidetes, Firmicutes, Cyanobacteria,Tenericutes, Actinobacteria, Planctomycetes, and Vercomicrobia, with dominant Proteobacteria in the intestines of Canadian rock scallops, accounting for 80%, and the subdominant Bacteroidetes, accounting for 15%. There were dominant Proteobacteria, accounting for 52%, and the subdominant Bacteroidetes, accounting for 18%, in the viscera of Canadian rock scallops. The dominant phylum Proteobacteria, accounting for 61%, and subdominant phylum Bacteroides, accounting for 26%, were observed in the intestine of the subgeneration rock scallops bred in domestic. The dominant phylum Proteobacteria, accounting for 73%, and the subdominant phylum Bacterioids, accounting for 17%, in the viscera in the first generation rock scallops. The findings indicated that there was rich in microorganism diversity in intestine and viscera of rock scallops, with to some extent, differences between Canadian rock scallop and subgeneration rock scallops bred in China.
邹建威, 曹善茂, 李晓雨, 刘钢, 王尧, 王斌. 岩扇贝原种和繁育后代肠道和内脏团细菌多样性分析[J]. 大连海洋大学学报, 2019, 34(3): 323-330.
ZOU Jian-wei, CAO Shan-mao, LI Xiao-yu, LIU Gang, WANG Rao, WANG Bin. Bacterial diversity in intestine and viscera of rock scallop Crassadoma gigantea. Journal of Dalian Ocean University, 2019, 34(3): 323-330.
2019, Vol. 34 
