Histopathology of ‘grey-white clinical symptoms of carapace’in three species of pet turtles
BAO Xiaoying1，PANG Wei1, PAN Liande1,2*, ZHANG Xiaoyu1
1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education，National Demonstration Center for Experimental Fisheries Science Education，Shanghai Ocean University，Shanghai；2.National Pathogen Library of Aquatic Animals，Shanghai Ocean University，Shanghai
Abstract: Clinical diagnosis and histopathology were investigated in six cases of ‘grey-white clinical symptoms of carapace’ disease in three species of aquatic pet turtles, Trachemysscriptaelegans, Mauremysreevesii and Podocnemiscayennensis by observation by naked eyes, Wood Lamp observation in darkroom, and paraffin section with H.E staining, Masson three-color dyeing and Perls blue dyeing in order to explore the clinical symptoms and pathological changes in ‘grey-white clinical symptoms of the carapace’ disease in the pet turtle.The results showed that clinical symptoms of the ‘grey-white clinical symptoms of the carapace’ diseased turtle were primarily characterized by the appearance of gray-white plaque on the carapace or plastron under dry condition, or gray and white color on whole shell in severe diseased turtles，and fluorescent under the Wood lamp in either case.The pathological anatomy, H.E stain，Masson stain and Perls blue stain of paraffin sections of the main organ tissues and the full-thickness ‘lesioned’ shell tissues indicate that the ‘grey-white clinical symptoms of the carapace’ disease makes the pet turtle suffering from necrosis and fragmentation of fibrillar keratin of the carapace lesion, eosinophil infiltration in the bone marrow space and spleen tissue, and intensive melanin deposition in tissues.For the artificial perforation of pet turtle shells, the establishment of dental resin(methyl methacrylate)seal repair, combined with intramuscular injection of gentamicin, can be applicable to related researches and applied in clinical treatment of shell diseases and the relative lesions.
鲍晓颖, 庞溦, 潘连德、, 张晓雨. 3种宠物龟“灰白甲”病组织病理学研究[J]. 大连海洋大学学报, 2020, 35(2): 205-212.
BAO Xiaoying, PANG Wei, PAN Liande, ZHANG Xiaoyu. Histopathology of ‘grey-white clinical symptoms of carapace’in three species of pet turtles. Journal of Dalian Ocean University, 2020, 35(2): 205-212.
2020, Vol. 35 
