Abstract: Manila clam Ruditapes philippinarum with body weight of(12.0±1.6)g and shell length of(3.0±0.35)cm were air exposed for 5 hours and stored in a refrigerator at 4℃ (group D-4℃)and at room temperature(group D-R)for 24 hours, and the air exposed for 5 hour clam were put directly into the aerated sea water as control group.The biochemical and metabolic parameters were analyzed in the clams in group D-4℃,group D-R and control group, including mass percentage of various parts of the clam,pH values of muscular and body cavity fluid,contents of water-soluble protein, muscular glycogen, ATP, AEC value and K value.The results showed that ATP was consumed in the clam during air exposure,and then the consumption was partially restored during wet storage(50 h).The mass of viscera were decreased slightly from 5.8%to 1.2%,and the mass of other soft tissues was kept stable during wet storage, almost unchanged in the water-soluble protein content.There was significant decrease in content of glycogen in the control group than that in the dry storage group, from 43.6 mg/g to 25.6 mg/g(P<0.05),with decrease in pH with the storage time in the storage(50 h).The muscular pH was found to be decreased from 7.0 to 6.7(P<0.05),and the pH value in the body cavity to be decreased from 7.7 to 7.3.The AEC values was kept from 71%to 76%,and the K value from 2%to 6%in the clam in the three groups.The good state of Manila clam during live wet storage(50 h)in this study indicates that short-term(24 h)air exposure will not affect the biochemical metabolism of Manila clam during live wet storage.