Abstract: The eukaryotic recombinant plasmid pEGFP－C1－HSP90 as an adjuvant was constructed on the basis of carp HPS90 mRNA gene sequence GenBank login，and then transfected into brain cells of common carp Cyprinus carpio(CCB)， being observed by fluorescent inverted microscope， in order to study the immune effect of HSP90.The antibody levels were detected in the common carp injected intramuscularly with the mixture of recombinant expression plasmid and pIRES－ORF81 at several immune doses by indirect enzyme－linked immunosorbent assay(ELISA).The results showed that serum injection of recombinant plasmid KHV was detected in the specific antibody in the common carp， with increase in the antibody level with the increase in immunization frequency， the peak in third and two weeks after immunization.There was higher antibody level in combined immunization than that in pIRES－ORF81 immunization alone， without significant difference(P＞0.05)， indicating that the recombinant plasmid pEGFP－C1－HSP90 as adjuvant of koi herpes virus has certain immune effects.
金晔, 袁海延, 王好, 周井祥, 刘艳辉, 李改娟, 祖岫杰. 鲤HSP90作为锦鲤疱疹病毒核酸疫苗免疫佐剂的效果研究[J]. 大连海洋大学学报, 2018, 33(2): 175-180.
JIN Ye, YUAN Hai－yan, WANG Hao, ZHOU Jing－xiang, LIU Yan－hui, LI Gai－juan, ZU Xiu－jie. Immune effect of carp HSP90 as an adjuvant of koi herpesvirus nucleic acid vaccine. Journal of Dalian Ocean University, 2018, 33(2): 175-180.
2018, Vol. 33 
