Abstract: Glycoprotein gene was cloned from the total RNA extracted from the suspension of Shlj1 strain with Spring Viremia of Carp Virus(SVCV)using RT-PCR,nucleotide sequence of the glycoprotein was sequenced, and amino acid was deduced to predict the spatial structure and B cell antigenic epitope of SVCV glycoprotein.A three dimensional model of the glycoprotein was established based on the amino acid sequence,and by Phyre2,and flexible region,hydrophilicity,surface probability and antigenic index of the glycoprotein was analyzed by the Protean module in DNAStar software.It was found that the glycoprotein of the SVCV Shlj1 strain had nucleotide sequences of 1527 bp coding 509 amino acids.Space structure prediction revealed that there were a certain number of glycoprotein α-helix,β-pleated sheet,β-turn and a large number of random coil structure,with relatively regular spatial confirmation,in the SVCV Shlj1 strain.The analysis of parameters such as antigen index and antigen epitope index indicated that there were many fragments with high antigen index located in the glycoprotein,with average antigen index of 1.025,and the maximum value of 1.250.The potential B cell antigenic epitope regions were more likely located in 4-26,145-157,293-302,315-327,and 407-491 amino acid sites,indicating that the SVCV Shlj1 strain appeared to have five antigen epitope regions located in the glycoprotein.The findings provide a theoretical basis for preparation of monoclonal antibody and design of epitope vaccine against SVCV.
2017, Vol. 32 
