Abstract: Identification,culture performance and nutrient analysis were conducted in a yeast Rhodotorula benthica by physiological and biochemical and molecular methods to explore the possibility of a yeast Rhodotorula benthica as dietary protein resource in aquaculture.It was found that the strain was similar to another Rhodotorula benthica in colony,cell morphology and physiological biochemical characteristics.Amplification and partially sequencing of ITS gene revealed that there was a 598 bp fragment in the total DNA sequence of the yeast,with 99%homology of nucleotide sequence with that in the reported Rhodotorula slooffiae ITS gene by NCBI GenBank,indicating that the yeast strain is Rhodotorula slooffiae.The peak value of 2.25×108cfu/mL and the maxiaml carotenoid concentration of 83.6 μg/g were observed in the liquid medium culture of the yeast strain.The findings indicate that Rhodotorula slooffiae is rich in nutrients including vitamins,unsaturated fatty acids and proteins,playing an important role in aquaculture in the future.
2017, Vol. 32 
