Abstract: The influence of dosage，solution pH value，diesel initial concentration and catalytic period on free horseradish peroxidase（HRP）degradation of marine diesel pollution were studied and optimized for the process conditions by orthogonal experiment to exploite utilization of horseradish peroxidase in the catalytic oxidation degradation of marine diesel pollution.The removal of marine diesel pollution by a reaction promoter was observed.The results showed that free horseradish peroxidase was shown to degradate the diesel pollution in the seawater effectively under the optimum conditions of diesel initial concentration of 1.2 g/L，temperature of 25℃，HRP activity concentration of 1.6 U/mL，H2O2mass concentration of 250 mg/L，pH 7.5 and reaction time of 3 h，with the diesel removal rate of 90.77%.It was found that polyethylene glycol（PEG）improved the efficiency for HRP catalytic oxidation in seawater diesel pollution.
2016, Vol. 31 
