Comparative proteome analysis of ovary and testis in tiger puffer Takifugu rubripes
LI Xin1, HU Ziwen1, WANG Shengnan1, QIU Xuemei1, LIU Ying1,2,WANG Xiuli1*
1.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;2.Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education,Dalian Ocean University, Dalian 116023, China
Abstract: The proteomes were analyzed qualitatively and quantitatively in gonads of 3 years old tiger puffer Takifugurubripes with body weight of 2.0 kg by lable-free mass spectrometry proteomics analysis, and the proteins of differentially expressed between the ovary and the testis were enriched using the methods of Gene ontology (GO) analysis and KEGG analysis. The results of lable-free quantification showed that there were 150 specific proteins in the ovary, 132 specific proteins in the testis, and 561 proteins common to the ovary and the testis, and 172 proteins with high expression level in the ovary and 161 proteins with high expression level in the testis were screened, with high expression level of ELAVL2 protein in the ovary associated with ovarian development and possibly the differential protein RNA helicase, LSM4, FRUYP1 contributed ovarian development. Furthermore, it was found that GSDF, as a protein associated with testis development, was specifically expressed in the testis. GO analysis showed that RNA binding affected ovarian development, with the two tissue-differential proteins having the maximal difference in integral component of membrane. The significant enrichment analysis of GO function showed that transferase activity was the most significant difference between the two tissues. KEGG pathway analysis showed that the ribosome pathway had the most differential proteins between the two tissues, the pathway being associated with gonadal development. The ribosomal protein L10A in the pathway was confirmed to be involved in the gender development of some species, and the phagocyte, lysosome and sphingolipid metabolic pathways were associated with sperm maturation. In this study, the differences in proteome between the ovary and testis were analyzed, proteins such as ELAVL2, RNA helicase, and ribosomal pathway related to ovarian development were found; and GSDF and pathways such as phagosome pathway related to testis development were observed. The findings provide a reference for the development of the gonad of tiger puffer.
李鑫, 胡子文, 王盛南, 仇雪梅, 刘鹰、, 王秀利. 红鳍东方鲀卵巢与精巢的蛋白质组比较分析[J]. 大连海洋大学学报, 2020, 35(6): 847-856.
LI Xin, HU Ziwen, WANG Shengnan, QIU Xuemei, LIU Ying, WANG Xiuli. Comparative proteome analysis of ovary and testis in tiger puffer Takifugu rubripes. Journal of Dalian Ocean University, 2020, 35(6): 847-856.
2020, Vol. 35 
