Establishment of SRAP reaction conditions and the genetic analysis of three strains with different shell colors in Manila clam Ruditapes philippinarum
JIN Di,QIN Yan-jie,YAN Xi-wu,LI Xia,ZHAO Li-qiang
Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China
Abstract: A SRAP-PCR system was established and the genetic diversity were analyzed in Manila clam Ruditapes philippinarum with white(W),black(B)and orange(O)shell colors through selective breeding by 17 pairs of SRAP primers screened from 36 primer combinations.A total of 510 loci were detected with 17 primer pairs,and Shannon's information index and Nei's gene diversity were found significant higher in W strain than those in B and O strain(P＜0.05),without significant differences between the last two strains.F-statistics(Gst)were varied from 0.000 1 to 0.644 8,with the mean value of 0.046 7,indicating that 4.67%of variations were observed between B and O strains,and 95.33%of variations within the populations.The gene flow were found between 0.275 4 and 2 000,with mean 10.202 7,and genetic distances between 0.028 2(W and B)and 0.039 6(W and O).The findings showed that there were weak genetic differentiations and strong gene flow among the three clam strains,and that the selective breeding for the clam was continued in order to establish new breeding.The 147 SRAP loci showing significant differences in frequency among the three strains(P＜0.05),and the 82 SRAP loci showing very significant differences in frequency among the three strains(P＜0.01),providing the foundation for explanation of the genetic basis of the good traits such as good growth,strong resistance and special shell colors in the three strains.
金迪, 秦艳杰, 闫喜武, 李霞, 赵力强. 菲律宾蛤仔SRAP标记体系的建立及不同壳色品系的遗传学分析[J]. 大连海洋大学学报, 2013, 28(4): 361-366.
JIN Di, QIN Yan-jie, YAN Xi-wu, LI Xia, ZHAO Li-qiang. Establishment of SRAP reaction conditions and the genetic analysis of three strains with different shell colors in Manila clam Ruditapes philippinarum. Journal of Dalian Ocean University, 2013, 28(4): 361-366.
2013, Vol. 28 
