Effects of different temperatures and light intensities on photosynthesis of green laver (Enteromorpha prolifera)
WANG Jingyi，WU Tingting，CAI Chuner*，LIU Yuling，ZHOU Lingjie，HE Peimin
Author information+
1.College of Oceanography and Ecological Science，Shanghai Ocean University，Shanghai 201306，China；2.Water Environment and Ecology Engineering Research Center，Shanghai Institution of Higher Education，Shanghai Ocean University，Shanghai 201306，China
{{custom_zuoZheDiZhi}}
{{custom_authorNodes}}
{{custom_bio.content}}
{{custom_bio.content}}
{{custom_authorNodes}}
Collapse
History+
Published
2024-12-18
Issue Date
2024-12-18
Abstract
In order to investigate the effects of temperature and light on the short-term interaction of photosynthesis in green laver (Enteromotphaprolifera) sampled at coastal Qingdao(120°11′24″ E, 36°2′24″ N), three temperature (4 ℃, 20 ℃, and 30 ℃) and three light intensities (20, 140, and 400 μmol/(m2·s)) interaction were evaluated, and the photosynthetic fluorescence parameters and differential gene expression were analyzed in the green laver treated for 24 h. The results showed that there was synergistic stress effect of short term high temperature and high light on the alga during the test cycle. The photochemical efficiency (Fv/Fm) of the alga was shown to be significantly reduced by high temperature and high light, with the down-regulated genes of the light trapping complex in high temperature and high light environments, and up-regulated in low temperature and low light. The alga attenuated the adverse environmental effects on the photosystems through up-regulation of the genes of the D1 protein, the CP43 protein and the manganese stabilising protein in the high temperature/high light and the low temperature/low light conditions, attenuating the negative regulatory effects of unfavourable environments on photosystem Ⅱ. The expression of genes encoding enzymes related to the C3 pathway was found to be down-regulated and the expression of genes related to the C4 pathway phosphoenolpyruvate carboxylase was up-regulated in high temperature and high light, effectively segregating O2 binding to ribulose-1,5-bisphosphate carboxylase/oxygenase, essentially eliminating photorespiration. The C4 pathway was the result of seaweed’s evolution to reduce the damage of photorespiration. It was shown that green laver was able to tolerate changes in low or high temperatures (4 ℃, 30 ℃) as well as low and high light [140 μmol/(m2·s), 400 μmol/(m2·s)], and withstanded environmental stresses through a variety of mechanisms. The findings may provide a useful reference for exploring the molecular mechanisms of green tide outbreaks in the Yellow Sea.
WANG Jingyi, WU Tingting, CAI Chuner, LIU Yuling, ZHOU Lingjie, HE Peimin.
Effects of different temperatures and light intensities on photosynthesis of green laver (Enteromorpha prolifera)[J]. Journal of Dalian Fisheries University, 2024, 39(5): 819-828 https://doi.org/10.16535/j.cnki.dlhyxb.2024-013
PDF(2355 KB)
