Journal of Dalian Ocean University  2023, Vol. 38 Issue (5): 874-884 DOI: 10.16535/j.cnki.dlhyxb.2023-014 |
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| Correlation analysis between metabolic characteristics and quality indices in muscle of Pacific white shrimp (Litopenaeus vannamei) during superchilling storage based on LC-MS non-targeted metabolomics |
| YANG Kaiying，PAN Chuang，HAO Shuxian，CHEN Shengjun，LIU Shucheng*，YANG Xianqing*
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| 1. Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety，College of Food Science and Technology，Guangdong Ocean University，Zhanjiang 524088，China；2.Key Laboratory of Aquatic Product Processing，Ministry of Agriculture and Rural Affairs，National Research and Development Center for Aquatic Product Processing，South China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510300，China；3.Collaborative Innovation Center of Seafood Deep Processing，Dalian Polytechnic University，Dalian 116034，China |
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Abstract To investigate the mechanism of quality deterioration of superchilled shrimp, metabolites change of Pacific white shrimp (Litopenaeus vannamei) during superchilling storage at -3 ℃ (0, 10, 20, 30 d) were investigated through liquid chromatography-mass spectrometry non targeted metabolomics. The key metabolites were screened by the correlation analysis between the potential biomarkers and the quality indices (including water holding capability, texture, pH, myofibrillar protein content, surface hydrophobicity and turbidity). The results showed that significant differences were observed in the whole metabolic composition of shrimp muscle during superchilling storage. According to the OPLS-DA model, 79 significant different metabolites were screened from the shrimp muscle at different superchilling storage times. These different metabolites notably altered with the extension of time. The main metabolic pathways involved were biosynthesis of amino acids, biosynthesis of alkaloids derived from histidine and purine, lysine biosynthesis, citrate cycle (TCA cycle) and biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid. The quality indices showed that water holding capability, hardness, springiness and myofibrillar protein content gradually decreased during storage, while pH, surface hydrophobicity and turbidity gradually increased. The pathway analysis combined with correlation analysis revealed that L-lysine, L-aspartic acid, citric acid and isocitric acid were used as the key metabolites related to the metabolites for the quality changes of shrimp muscle during superchilling storage. This study revealed the metabolic characteristics of superchilled shrimp muscle at the molecular level, providing a theoretical basis for quickly and accurately reflecting the actual-time quality of shrimp muscle.
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Published: 08 November 2023
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