Sequence characteristics, SNP locus and association with growth traits of insulin-induced gene 1 (INSIG1) in Yellow River carp (Cyprinus carpio haematoperus)
WANG Xinhua, LU Chang, XU Wenyan, QI Zixin, XU Mengmeng
Sequence characteristics, SNP locus and association with growth traits of insulin-induced gene 1 (INSIG1) in Yellow River carp (Cyprinus carpio haematoperus)
WANG Xinhua，LU Chang，XU Wenyan，QI Zixin*，XU Mengmeng
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1.College of Animal Science and Technology，Henan Academy of Animal Husbandry and Economics，Zhengzhou 450046，China；2.Fisheries College，Ocean University of China，Qingdao 266000，China
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Published
2023-11-08
Issue Date
2023-11-08
Abstract
In order to explore the function of insulin-induced gene 1 (INSIG1) in the growth and development process of Yellow River carp (Cyprinuscarpiohaematoperus), the sequence characteristics, expression patterns and SNP association with growth traits of INSIG1 in 26 all-sibling lineages of Yellow River carp (Cc-INSIG1) were analyzed based on the candidate gene approach. The results showed that the full-length DNA of Cc-INSIG1 gene was 4 853 bp, containing 5 exons and 4 introns, and the full-length cDNA of Cc-INSIG1 gene was 1 530 bp, including 756 bp of the coding sequence, encoding 251 amino acids. The amino acid sequence of Cc-INSIG1 showed high homology with INSIG1 of other species, and clustered with other fish species in Cyprinidae like horned golden-line barbel (Sinocyclocheilusrhinocerous), zebrafish (Daniorerio) and crucian carp (Carassiusauratus). The Cc-INSIG1 protein had relative molecular mass of 27 674.25 and the theoretical isoelectric point (pI) of 8.09, and belonged to the stable hydrophobic protein, without signal peptide. The structure analysis revealed that there were 6 transmembrane structures and the tertiary structure mainly consisted of 6 alpha helices and random coils. Real-time PCR analysis showed that Cc-INSIG1 was ubiquitous expressed in liver, spleen, kidney, intestine, heart, gill, brain, muscle and skin, with extreme predominance in intestine and liver, and the minimum in gill. Ten single-nucleotide polymorphisms (SNP) were identified in DNA sequence of Cc-INSIG1, two, that is, g.1854T>C and g.1982A>T, were significantly associated with Fulton’s condition factor (K) in the populations of Yellow River carp (P<0.05), and without association with growth traits in other SNP. It was found that INSIG1 gene was involved in the growth and development of Yellow River carp, and its mutation sites g.1854T>C and g.1982A>T significantly influenced the Fulton’s condition factor of Yellow River carp. The findings provided available markers for gene (marker)-assisted selective breeding in Yellow River carp, which has great significance to the breeding of Yellow River carp with excellent economic traits.
WANG Xinhua, LU Chang, XU Wenyan, QI Zixin, XU Mengmeng.
Sequence characteristics, SNP locus and association with growth traits of insulin-induced gene 1 (INSIG1) in Yellow River carp (Cyprinus carpio haematoperus)[J]. Journal of Dalian Fisheries University, 2023, 38(5): 763-771 https://doi.org/10.16535/j.cnki.dlhyxb.2023-001
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