Structural characterization and the synergistic effects with anthocyanin of oyster hypoglycemic peptides

doi:10.16535/j.cnki.dlhyxb.2022-282

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Journal of Dalian Fisheries University ›› 2023, Vol. 38 ›› Issue (3) : 455-463. DOI: 10.16535/j.cnki.dlhyxb.2022-282

Structural characterization and the synergistic effects with anthocyanin of oyster hypoglycemic peptides

JIANG Meiling，CHEN Zhongqin*，QIN Xiaoming*，SUN Xujia，CAO Wenhong，LIN Haisheng，GAO Jialong

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Abstract

To investigate the hypoglycemic effects and digestive stability of oyster peptides, the oyster peptides were prepared from Hong Kong oyster (Crassostrea hongkongensi). Structural characteristics, hypoglycemic activity and digestive stability of prepared oyster peptides were investigated in vitro by hypoglycemic model and simulated gastrointestinal digestion model. The anthocyanin was also used to improve the digestive stability and hypoglycemic activity of prepared oyster peptides. The results showed that the prepared oyster peptides were rich in hydrophobic amino acids, and the relative molecular mass was mainly less than 1 000. The spectrum analysis showed that prepared oyster peptides contained 15 peptide sequences mainly including LYF, TLFLK, IRAGYD, TLHHRVH, ARNEANVNIY, and CVIGR, which had typical structure characteristics of hypoglycemic peptides. The half inhibition concentration (IC50) of prepared oyster peptides against α-amylase, α-glucosidase and dipeptidyl peptidase Ⅳ (DPP-Ⅳ) was (3.66±0.47), (8.62±0.66) and (2.60±0.46) mg/mL, respectively, exhibiting good hypoglycemic activity. However, the digestive stability of prepared oyster peptides was weak. After simulated gastrointestinal digestion in vitro, the inhibition rate against the above three enzymes significantly decreased by 6.23%-32.48% (P<0.05). Therefore, the synergistic effects of anthocyanin with prepared oyster peptides were investigated. Through the synergy matrix design, the highest single agent (HSA) model was selected to calculate the synergistic effects of anthocyanin with prepared oyster peptides. The results showed that the oyster peptide and anthocyanin complex had significant synergistic inhibitory effects against α-amylase (HSA synergistic score 3.632), α-glucosidase (HSA synergistic score 3.503) and DPP-Ⅳ (HSA synergistic score 3.156). Moreover, the results of simulated gastrointestinal digestion experiment in vitro showed that the anthocyanin could improve the digestive stability of prepared oyster peptides. The results of this study indicate that oyster peptides have the characteristic structure of hypoglycemic peptides, and oyster peptides and anthocyanins have synergistic effect in enhancing hypoglycemic activity and digestive stability.

Key words

oyster peptide / structural characterization / hypoglycemic activity / anthocyanin / synergistic effect

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JIANG Meiling, CHEN Zhongqin, QIN Xiaoming, SUN Xujia, CAO Wenhong, LIN Haisheng, GAO Jialong. Structural characterization and the synergistic effects with anthocyanin of oyster hypoglycemic peptides[J]. Journal of Dalian Fisheries University, 2023, 38(3): 455-463 https://doi.org/10.16535/j.cnki.dlhyxb.2022-282