Journal of Dalian Ocean University  2023, Vol. 38 Issue (3): 406-413 DOI: 10.16535/j.cnki.dlhyxb.2022-264 |
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| α-Tubulin gene structure, SNP screening and its correlation with low temperature tolerance in the pearl oyster (Pinctada fucata martensii) |
| LIU Ya，LI Songyu，LAI Zhuoxin，ZHONG Ruzhuo，WANG Qingheng*，DENG Yuewen
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| 1.Zhanjiang Institute of Clinical Medicine，Central People’s Hospital of Zhanjiang，Zhanjiang 524000，China；2.Fisheries College，Guangdong Ocean University，Zhanjiang 524088，China；3.Guangdong Science and Innovation Center for Pearl Culture，Zhanjiang 524088，China |
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Abstract In order to explore the function of α-Tubulin gene of the pearl oyster (Pinctada fucata martensii) in low temperature adaptability, ɑ-Tubulin gene of pearl oyster was identified in this study, the expression trend of ɑ-Tubulin was analyzed in response to low-temperature stress, and the SNP sites in the ɑ-Tubulin coding region of pearl oyster were screened and compared between the low temperature resistan line (R) F3 and the Beibu Gulf wild population (W). The results showed that α-Tubulin has a total length of 2 107 bp, encoding a 454 amino acid protein containing typical Tubulin and Tubulin-C domains. Whole-tissue quantification showed that ɑ-Tubulin was expressed in all examined tissues, with the highest expression in the foot (P<0.05). Under low temperature stress, ɑ-Tubulin relative expression in gill in the low temperature group (12 and 17 ℃) reached the highest value at 72 h, which was significantly higher than that in the control group (22 ℃)(P<0.05). There were 34 SNP in the ɑ-Tubulin exon region, and the genotype frequencies of 3 SNP were significantly different between the R and W populations (P<0.05). Linkage disequilibrium analysis showed that the frequency of haplotype CCTCAGCGCC in the R population was significantly higher than that in the W population. All data observed in this research show that ɑ-Tubulin might be a candidate gene involved in regulating the adaptation to low temperature of the pearl oyster. The SNP site g.111071125 and excellent haplotype CCTCAGCGCC related to low temperature resistance could be selected as candidate markers for selective breeding.
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Published: 12 July 2023
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