Journal of Dalian Ocean University  2023, Vol. 38 Issue (3): 397-405 DOI: 10.16535/j.cnki.dlhyxb.2022-241 |
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| Effects of short-term feeding with water extract of pomegranate peel on Edwardsiella piscicida infection prevention and control in sea bass (Lateolabrax maculatus) |
| HU Jianmei，MA Zhuang，WANG Baotun，HUANG Weimin，FENG Juan，SU Youlu*
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| 1.Innovative Institute of Animal Healthy Breeding，College of Animal Sciences and Technology，Zhongkai University of Agriculture and Engineering，Guangzhou 510225，China；2.Key Laboratory of South China Sea Fishery Resources Development &Utilization，Ministry of Agriculture and Rural Affarrs，South China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510300，China；3.National Demonstration Center for Experimental Fisheries Science Education，Shanghai Ocean University，Shanghai 201306，China |
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Abstract In order to explore the effects of short-term feeding sea bass (Lateolabrax maculatus) with water extract of pomegranate peel (Punica granatum L.) on Edwardsiella piscicida infection prevention and control. The water extract of pomegranate peel was added to the daily diet (4 and 8 g/kg) and fed sea bass (body weight 10.14 g±2.53 g) continuously for 7 days. Physiological and biochemical indices of serum, activities of immune related enzymes in serum and the relative expression of immune related genes were determined at the 3, 5 and 7 d after feeding. Meanwhile, artificial pathogen immersion challenge experiment was performed to investigate the effects of water extract of pomegranate peel on E.piscicida infection prevention and control. The results showed that the contents of serum total cholesterol, glutamic pyruvic transaminase, glutathione reductase, lactate dehydrogenase, total protein and high density lipoprotein in the experimental group fed with water extract of pomegranate peel were significantly higher than those in the control group (P<0.05). After 3 and 5 d of feeding, the content of serum blood glucose decreased significantly (P<0.05). The relative expression of serum alkaline phosphatase (AKP) gene in the experimental group increased significantly (P<0.05). The relative expression of LZM gene in spleen and head kidney and NK-lysin in head kidney decreased. And the relative expression of other examined immune related genes increased. The survival rate of infected fish was significantly increased after feeding with water extract of pomegranate peel for 5 d (P<0.05) indicating water extract of pomegranate peel has good effects on E.piscicida infection prevention and control. The relative protection ratio in 4 g/kg and 8 g/kg experimental groups was 50% and 47%, respectively. The results showed that short-term feeding with water extract of pomegranate peel could enhance the immunity and disease resistance of sea bass, and provide a reference for E.piscicida infection prevention and control.
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Published: 12 July 2023
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HUANG Hua, YU Guanglei, WANG Liyong, GAO Yan, ZHANG Lan, SUN Baiming, ZHUANG Yanwen, WANG He. Isolation, identification, and analysis of virulence gene and drug resistance gene in pathogenic Edwardsiella piscicida from cultured turbot Scophthalmus maximus[J]. Journal of Dalian Ocean University, 2022, 37(6): 922-932. |
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