Isolation, identification and denitrification performance of a salt-tolerant and efficient aerobic denitrifier Halomonas sp.HRL-11

doi:10.16535/j.cnki.dlhyxb.2022-015

PDF(2131 KB)

Journal of Dalian Fisheries University ›› 2022, Vol. 37 ›› Issue (2) : 227-234. DOI: 10.16535/j.cnki.dlhyxb.2022-015

Isolation, identification and denitrification performance of a salt-tolerant and efficient aerobic denitrifier Halomonas sp.HRL-11

MA Hongjing, LIU Ying, MU Xinting, SU Xin, SONG Jing, WU Yinghai, HAN Rui

Author information +

History +

Abstract

In order to obtain an efficient aerobic denitrifier and ascertain the aerobic denitrification capacity, a salt-tolerant and efficient aerobic denitrifier was isolated from seawater in recirculating aquaculture systems(RAS).The effects of dissolved oxygen(5.58, 6.87, and 7.48 mg/L regulated by various speeds of 90, 120 and 150 r/min), C/N ratio(3, 5, 10, and 15)and temperature(20, 30 and 40 ℃)on the aerobic denitrification performance and nitrogen conversion pathway were investigated.The results showed that the isolated aerobic denitrifier was identified as Halomonas sp.HRL-11.At high salinity(31), the optimal nitrate nitrogen reduction by HRL-11 was observed under conditions of 150 r/min(initial DO concentration 7.48 mg/L), C/N 10 and 30 ℃, with 100 mg/L

as the sole nitrogen source by HRL-11, the OD600nm value of 1.4 in 48 h, the removal efficiency of 91.5% for

, and the removal efficiency of 69.4% for TOC and the removal efficiency of 65.6% for TN.The nitrogen balance analysis revealed that 19.44% of

was transformed into intracellular nitrogen, 25.87% of

was transformed into other nitrification products

and organic nitrogen), and 46.19% of

was transformed into gaseous products by Halomonas sp.The findings indicate that Halomonas sp.HRL-11 has excellent salt-tolerance and aerobic denitrification performance and the potential application to treat high-salinity wastewater in industrialized seawater RAS.

Key words

aerobic denitrification / salt tolerance / denitrification / mariculture wastewater

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

MA Hongjing, LIU Ying, MU Xinting, SU Xin, SONG Jing, WU Yinghai, HAN Rui. Isolation, identification and denitrification performance of a salt-tolerant and efficient aerobic denitrifier Halomonas sp.HRL-11[J]. Journal of Dalian Fisheries University, 2022, 37(2): 227-234 https://doi.org/10.16535/j.cnki.dlhyxb.2022-015