Effects of low temperature stress on survival and expression of fatty acid metabolism-related genes SCD，FAD and ACC indifferent populations of Manila clam Ruditapes philippinarum

doi:10.16535/j.cnki.dlhyxb.2021-243

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Journal of Dalian Fisheries University ›› 2022, Vol. 37 ›› Issue (4) : 636-642. DOI: 10.16535/j.cnki.dlhyxb.2021-243

Effects of low temperature stress on survival and expression of fatty acid metabolism-related genes SCD，FAD and ACC indifferent populations of Manila clam Ruditapes philippinarum

LI Ning, NIE Hongtao*, LI Qiang, HUO Zhongming, YAN Xiwu*

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Abstract

In order to probe into the effects of low temperature stress on the survival and fatty acid metabolism related genes of Manila clam Ruditapes philippinarum zebra clam No.2, the survival differences and three genes related to fatty acid metabolisms including stearoyl coenzyme A desaturase (SCD), fatty acid desaturase (FAD) and acetyl coenzyme A carboxylase (ACC) in gill tissues were investigated in three populations including zebra clam No.2, Dalian in Liaoning Province and Beihai in Guangxi under low temperature stress of -1 (in a refrigerator)， 4 (in a recirculating tank)， and 12 ℃ (room temperature) by qRT-PCR. The results showed that after acute low temperature (-1, 4 ℃) stress for 9 d, the order of the survival rates of Manila clam populations was expressed as zebra clam No.2 > Dalian population in Liaoning > Beihai population in Guangxi. The expression of three fatty acid metabolism related genes in gill tissues was significantly affected by temperature (P<0.05 ).With the temperature decreasing from 12 ℃ to -1 ℃, the expression level of the three genes were increased under 4 ℃ or -1 ℃ cold stress. Compared with 0 h, the expression levels of SCD and FAD genes in gill tissues of zebra clam No.2, Beihai population and Dalian population were significantly up-regulated from 12 to 72 h(P<0.05). The expression levels of ACC genes in the three populations were significantly up-regulated at different time, and the up-regulation range of the three genes in the gills of zebra clam No.2 was significantly higher than that in Beihai population and Dalian population (P<0.05). The results demonstrated that the survival rate and low temperature tolerance of zebra clam No.2 at -1 ℃ were apparently higher than Dalian, Liaoning and Beihai, Guangxi. In addition, the high expression level of SCD, FAD and ACC genes of zebra clam No.2 might be related to its strong tolerance and high resistance to low temperature.

Key words

Ruditapes philippinarum / cold stress / survival rate / fatty acid metabolism / gene expression

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LI Ning, NIE Hongtao, LI Qiang, HUO Zhongming, YAN Xiwu. Effects of low temperature stress on survival and expression of fatty acid metabolism-related genes SCD，FAD and ACC indifferent populations of Manila clam Ruditapes philippinarum[J]. Journal of Dalian Fisheries University, 2022, 37(4): 636-642 https://doi.org/10.16535/j.cnki.dlhyxb.2021-243