Isolating culturable endophytic bacteria from sea grass Suaeda salsa leaves and screening the potential plant growth promoting bacterium

doi:10.16535/j.cnki.dlhyxb.2021-232

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Journal of Dalian Fisheries University ›› 2022, Vol. 37 ›› Issue (2) : 235-243. DOI: 10.16535/j.cnki.dlhyxb.2021-232

Isolating culturable endophytic bacteria from sea grass Suaeda salsa leaves and screening the potential plant growth promoting bacterium

SHI Jia, XU Shuang, WANG Bo, XIE Xiaochen, LI Chen, FANG Lei*

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Abstract

With an attempt to screen sea grass Suaeda salsa plant growth promoting bacteria, six strains of culturable endophytic bacteria were isolated from the plant leaves and then subject to preliminary molecular identification and in vitro plating experiments to examine the abilities of dissolving phosphorus, decomposing potassium, fixing nitrogen, producing iron carriers and generating ACC deaminase.Colonization experiments using hydroponically grown seedlings were also performed to validate the plant growth promoting abilities of those bacteria.The results showed that through continuous plate streaking using 2216E agar plates, 6 strains of culturable bacteria were isolated from the plant leaves, among which 4 strains had the ability of producing iron carriers(labeled as JY21, JY24, BJY223 and BJY323, respectively)and 2 strains had the ability of generating ACC deaminase(labeled as JY32 and BJY323, respectively).A potential plant growth promoting bacterium(labeled as BJY223)was screened through colonization experiments, which could very significantly promote root elongation as well as stem growth of the hydroponically grown the seedlings(P<0.01).A BLASTn search against the NCBI nucleotide collection using the full sequence of the 16S rRNA gene of BJY223 was carried out to dig out its homologous bacteria, based on which a phylogenetic tree was plotted.BJY223 was therefore identified as being most closely related to bacteria of genus Pseudoalteromonas.The in vitro plating experiments as well as in vivo seedling colonization experiments revealed that a potential plant growth promoting bacterium was isolated from the S.salsa leaves, which will help to enrich the resources of potential S.salsa growth promoting bacteria.

Key words

Suaeda salsa / Pseudoalteromonas / plant endophytic bacterium / plant growth promoting bacterium

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SHI Jia, XU Shuang, WANG Bo, XIE Xiaochen, LI Chen, FANG Lei. Isolating culturable endophytic bacteria from sea grass Suaeda salsa leaves and screening the potential plant growth promoting bacterium[J]. Journal of Dalian Fisheries University, 2022, 37(2): 235-243 https://doi.org/10.16535/j.cnki.dlhyxb.2021-232