Effects of pathogen Vibrio harveyi infection on DNA methylation of IL-6 gene in tiger puffer Takifugu rubripes

doi:10.16535/j.cnki.dlhyxb.2021-115

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Journal of Dalian Fisheries University ›› 2022, Vol. 37 ›› Issue (2) : 221-226. DOI: 10.16535/j.cnki.dlhyxb.2021-115

Effects of pathogen Vibrio harveyi infection on DNA methylation of IL-6 gene in tiger puffer Takifugu rubripes

WANG Chenshi, HUANG Xindi, CUI Xiaoyu, NI Ping, YE Shigen, WANG Hua, GAO Dongxu, LEI Wei

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Abstract

To investigate effect of pathogen Vibrio harveyi infection on DNA methylation of the interleukin-6(IL-6)gene in tiger puffer Takifugu rubripes, tiger puffer with body mass of(100.5±10.8)g was exposed to clean sea water(control group)and V.harveyi(107 cfu/mL)(treatment group)for 12 h and then transferred to clean sea water without pathogens for 7 d when histological changes,and the relative expression level of IL-6, and DNA methylation levels and patterns in the IL-6 promoter region were determined in the spleen of tiger puffer.The results showed that there was a large degree of cell necrosis in the diseased group, and no obvious necrotic areas were found in the non-diseased group.There was significantly higher mRNA level of IL-6 in the spleen in the diseased group than that in the control and non-diseased groups(P<0.05), without significant difference between the control and non-diseased groups(P>0.05).The methylation level of IL-6 was shown to be significantly higher in the spleen of the non-diseased group than that in the diseased group(P<0.05).These findings indicated that tiger puffer chalenged with V.harveyi showed different degrees of disease resistance, which might be involved in significantly different methylation states at the IL-6 promoter.

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aquaculture / Takifugu rubripes / Vibrio harveyi / IL-6 / DNA methylation

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WANG Chenshi, HUANG Xindi, CUI Xiaoyu, NI Ping, YE Shigen, WANG Hua, GAO Dongxu, LEI Wei. Effects of pathogen Vibrio harveyi infection on DNA methylation of IL-6 gene in tiger puffer Takifugu rubripes[J]. Journal of Dalian Fisheries University, 2022, 37(2): 221-226 https://doi.org/10.16535/j.cnki.dlhyxb.2021-115