Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus)
LIU Zhigang，GAO Fengying，TONG Yannan，YI Mengmeng，WANG Miao，ZHU Hai，LU Maixin，KE Xiaoli*，CAO Jianmeng*
1.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation，Ministry of Agriculture and Rural Affairs，Guangdong Provincial Key Laboratory of Aquatic Animal Immune Technology，Pearl River Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510380，China；2.College of Fisheries and Life Sciences，Shanghai Ocean University，Shanghai 201306，China；3.Hainan Academy of Ocean and Fisheries Science，Haikou 570206，China
Abstract: In order to screen growth-related microsatellite markers for breeding in Nile tilapia (Oreochromisniloticus), the relationship between the growth traits (body weight, total length, body length, head length, body height and body width)and genotypes was analyzed in four tilapia populations (Gaoyao F0 population, Gaoyao F1 population, Panyu population and Hainan population) by 29 microsatellite markers. The results showed that a total of 242 alleles were detected at the 29 microsatellite loci in Gaoyao F0 population, with mean allele number (Na) of 8.345, observed heterozygosity (Ho) of 0.621 and polymorphism information content (PIC) of 0.842. The PIC values of all the loci were found to be great than 0.5, indicating that the genetic diversity was in high level in Gaoyao F0 population. Six microsatellite markers (GM114, GM213, GM423, GM642, UNH130 and UNH985) had significant correlation with more than one growth trait in Gaoyao F0 population (P<0.05), in which four microsatellite markers (GM213, GM642, UNH130 and UNH985) were significantly correlated with the growth traits in Gaoyao F1 population (P<0.05).It was found that these markers were genetically stable in different generations. The validation of the preliminary screening growth-related microsatellite markers in two out groups (Panyu population and Hainan population) revealed that four loci (GM213, GM642, UNH130 and UNH985) and three loci (GM642, UNH130 and UNH985) showed significant difference in growth traits among different genotypes in Panyu population and Hainan population (P<0.05). In conclusion, GM642, UNH130 and UNH985 were shown to be significantly correlated with growth traits of tilapia in different generations and populations. Tilapia with DD and HH genotypes of GM642, AD, BC and FF genotypes of UNH130, and GH and CF genotypes of UNH985 have obvious growth advantage, which can be regarded as candidate markers for Nile tilapia growth.
刘志刚, 高风英, 佟延南, 衣萌萌, 王淼, 朱海, 卢迈新, 可小丽, 曹建萌. 尼罗罗非鱼生长性状相关微卫星标记的筛选与验证[J]. 大连海洋大学学报, 2023, 38(6): 925-934.
LIU Zhigang, GAO Fengying, TONG Yannan, YI Mengmeng, WANG Miao, ZHU Hai, LU Maixin, KE Xiaoli, CAO Jianmeng. Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 2023, 38(6): 925-934.
2023, Vol. 38 
