Abstract: To investigate the pathogen responsible for the intussusception disease of half-smooth tongue sole Cynoglossussemilaevis Günther,parasite inspection,pathogen isolation,histopathological analysis,infection test,physiological and biochemical tests,and drug susceptibility tests were performed in 10 diseased fish with body length of 10-15 cm collected from a breeding plant in Tianjin in September 2020 by 16S rDNA gene sequence analysis.The results showed that there were no large numbers of parasites on the body surfaces of the diseased fish,whereas a large number of nodules were found in the spleens,with decayed and fallen away intestinal mucosal tissue and intestinal villi.The dominant bacterial strain ST-2020-2 was isolated from the kidney caused morbidity in healthy half-smooth tongue sole,with 90% mortality in the infection test.Integrating the physiological and biochemical characteristics and the 16S rDNA gene sequence analysis revealed that ST-2020-2 was identified as strain Photobacteriumdamselae subsp.piscicida and sensitive to streptomycin,ciprofloxacin and florfenicol,moderately sensitive to tetracycline,kanamycin and ampicillin,and resistant to 10 drugs,including neomycin,polymyxin B,and tobramycin.The findings indicated that Photobacteriummermaid was the pathogen of intestinal disease observed in the half-smooth tongue sole,and that provided essential data for the prevention and control of this disease.
何晓旭, 刘克明, 汪笑宇, 郝爽, 贾磊, 邵蓬, 罗璋. 半滑舌鳎套肠症病原的分离鉴定和组织病理观察[J]. 大连海洋大学学报, 2022, 37(1): 19-25.
HE Xiaoxu, LIU Keming, WANG Xiaoyu, HAO Shuang, JIA Lei, SHAO Peng, LUO Zhang. Isolation,identification and histopathology of pathogen of intussusception disease in half-smooth tongue sole Cynoglossu semilaevis. Journal of Dalian Ocean University, 2022, 37(1): 19-25.
2022, Vol. 37 
