Abstract: The effects of temperatures(5-40℃)on cell density,relative chlorophyll content and chlorophyll fluorescence parameters(maximal effective quantum yield of PSⅡin the dark,Fv/Fm;the photochemical quenching coefficient qP)were examined in Tahitian strain of Isochrysis galbana in batch cultures by Water-PAM chlorophyll fluorometer.One-way analysis of variance showed that temperature had significant effects on the cell density and relative chlorophyll content of the alga(Tahitian)during the experiment(P＜0.05).Under this experimental condition,the proper growth temperature for the alga was 15-35℃,the optimal growth temperature of 20-30℃.The alga was found to cease growing and developing under 5,10℃and 40℃.The relative growth ratio under different temperatures was arranged as 25℃＞20℃＞30℃＞35℃＞15℃.Temperature also had significant effects on the fluorescence parameters of the alga.The main parameters(Fv/Fm,qP)were significantly lower under 5,10℃and 40℃than those in other treatments from day 1 to the end of this experiment.At 25℃and 30℃,however,the above main fluorescence parameters were generally lower than those at 15℃,20℃and 35℃,but higher than others treatments.After cultured at 5,10℃and 40℃for ten days,the microalga was re-cultured in 25℃,100 μmol/(m2·s)conditions for 6 days,all the parameters in 5℃and 40℃treatment were not recovered absolutely, while those in 10℃treatment were recovered to the normal level.The cell density,relative chlorophyll content and fluorescence parameters exhibited some correlations with temperatures,while the pattern and strength were varied with the temperature range and the culture period.Moreover,significant positive correlations between chlorophyll content and cell density were found during the experiment.
梁英, 范丽敏, 陈书秀, 王燕. 温度对等鞭金藻塔溪堤品系细胞密度和叶绿素荧光参数的影响[J]. 大连海洋大学学报, 2011, 26(5): 422-427.
LIANG Ying, FAN Li-min, CHEN Shu-xiu, WANG Yan. Effects of temperature on cell density and chlorophyll fluorescence parameters in Tahitian strain of alga Isochrysis galbana. Journal of Dalian Ocean University, 2011, 26(5): 422-427.
2011, Vol. 26 
