Abstract: Chinese sleeper Perccottusglenii(body weight of 30.0 g± 2.7 g)acclimated at water temperature of 4 ℃ were moved to a transparent food preservation box in a refrigerator at density of 10 fish per box at icing up at a cooling rate of 1 ℃/h for 24-48 h to investigate tolerant ability of Chinese sleeper, and physiological and biochemical indictors were determined in the survived Chinese sleeper during pre-icing[0 ℃, dissolved oxygen(DO)level of 6 mg/L, group T1], icing up(24 h, -2 ℃, DO level of 0 mg/L, group T2)and fish recovery(4 ℃, DO level of 6 mg/L, 1 h recovery group T3, 24 h recovery, group T4, and 72 h recover, group T5)to evaluate physiological and biochemical responses of Chinese sleeper to icing and hypoxia environment.The results showed that the Chinese sleeper had survival of 100% in ice at -2 ℃ for 24 h, and decreased to 0% at -5 ℃ for 24 h with the ice temperature decrease.Compared to the indicators of Chinese sleeper during the pre-freezing period, liver glycogen content was decreased significantly(P<0.05), the muscle glycogen level was decreased without significant difference(P>0.05), and glucose contents in heart, brain, gill and muscle were significantly increased(P<0.05), and lactic acid content in gill, brain, heart increased significantly in the periods of freezing and fish recovery(P<0.05), without significant differences in activities of lactic dehydrogenase in various tissues(P>0.05).The findings indicate that Chinese sleeper can tolerant mild icing up completely and anoxia environment for a short time, and deal with icing up and hypoxia environment depending on glucose as major cryoprotectant by decomposing liver glycogen, and enhancing metabolism of anaerobic glycolysis.
柴龙会, 黄璞祎, 暴旭. 葛氏鲈塘鳢耐受完全结冰缺氧环境的能力及生理生化响应[J]. 大连海洋大学学报, 2020, 35(2): 218-222.
CHAI Longhui, HUANG Puyi, BAO Xu. Tolerant ability and physiological and biochemical responses of Chinese sleeper Perccottus glenii to icing up and hypoxia environment. Journal of Dalian Ocean University, 2020, 35(2): 218-222.
2020, Vol. 35 
